Nestin anticorps

N° du produit ABIN112422

Cet anticorps anti-Nestin est un anticorps Souris Monoclonal détectant Nestin dans WB, IF, IHC (p) et IHC (fro). Adapté pour Souris et Rat.

Aperçu rapide pour Nestin anticorps (ABIN112422)

Antigène: Nestin (NES)

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nestin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 4D4 (Rat-401)

Détail du produit anti-Nestin anticorps

Specificité: This antibody recognizes Mouse and Rat Nestin and does not recognizes Human Nestin.

Purification: Ig Fraction

Immunogène: Nestin purified from embryonic rat spinal cord. Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.

Isotype: IgG

Information d'application

Indications d'application: Western Blot: reacts with a band at 200-220 kDa in reducing gels of newborn rat or mousecell extracts, For the purpose of western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10 % 2-mercaptoethanol, 10 % glycerol and4.6 % SDS, Membranes should be blocked with milk or BSA, 5 % PAGE gels are suggested. Immunohistochemistry on frozen and paraffin sections: 1/20 - 1/200, We recommendperfusing tissues with 4 % paraformaldehyde at pH 7.4 for light microscopy or with either4 % paraformaldehyde at pH 10.0 or 4 % paraformaldehyde with 0.1 % glutaraldehyde at pH 7.4 for EM, For Immunocytochemistry we recommend using cells fixed in 4 %paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: TRIS buffered saline, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Nestin

Antigène: Nestin (NES)

Autre désignation: Nestin

Sujet: Nestin is a large intermediate filament protein originally identified by Hockfield and McKay (1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas. This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.Synonyms: NES, Nbla00170

ID gène: 18008

NCBI Accession: NP_057910

UniProt: Q6P5H2