TRAIL anticorps

N° du produit ABIN115786

Détail du produit anti-TRAIL anticorps

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TRAIL est non-conjugé

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Specificité: Recognises Human TRAIL/APO-II.

Purification: Affinity Chromatography on Protein A

Immunogène: Recombinant Human TRAIL/APO-II

Isotype: IgG1

Information d'application

Indications d'application: ELISA: In a Sandwich ELISA (assuming 100 μL/well), a concentration of 1-2 μg/mL of thisantibody will detect at least 0.2 ng/well of recombinant Human TRAIL/APO-II when usedwith Biotin conjugated anti-Human TRAIL/APO-II (cat. PP1084B) as the detection antibody. Western Blot: To detect Human TRAIL/Apo2L by Western Blot analysis this antibody can beused at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant Human TRAIL/Apo2L is 0.25-1.0 ng/lane, under reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman TRAIL/Apo2L (10.0 ng/mL), a concentration of 0.30-0.60 μg/mL of this antibody isrequired.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Concentration: 1.0 mg/mL

Buffer: PBS without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur TRAIL

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Autre désignation: CD253 / TRAIL (TNFSF10 Produits)

Synonymes: anticorps APO2L, anticorps Apo-2L, anticorps CD253, anticorps TL2, anticorps TRAIL, anticorps A330042I21Rik, anticorps AI448571, anticorps APO-2L, anticorps Ly81, anticorps Trail, anticorps tl2, anticorps apo2l, anticorps cd253, anticorps trail, anticorps apo-2l, anticorps trail1, anticorps xtrail1, anticorps tnfsf10l2, anticorps zgc:92320, anticorps TNLG6A, anticorps TNF superfamily member 10, anticorps tumor necrosis factor (ligand) superfamily, member 10, anticorps tumor necrosis factor superfamily member 10, anticorps TNF superfamily member 10 S homeolog, anticorps TNFSF10, anticorps Tnfsf10, anticorps tnfsf10.S, anticorps tnfsf10

Sujet: Apoptosis or programmed cell death is induced in cells by a group of death domain containing receptors. Binding of ligand to these receptors sends signals that activate members of the caspase family of proteases. The signals ultimately cause degradation of chromosomal DNA by activating DNase. TRAIL (TNF related apoptosis induced ligand) or Apo 2L initiates apoptosis of tumor cells by binding to either of its receptors, DR4 or DR5. These receptors consist of an extracellular TRAIL binding domain and a cytoplasmic "death domain". In addition, two decoy receptors for TRAIL have also been identified. These receptors, designated DcR1 and DcR2, lack the death domain. Binding of TRAIL to either of these receptors, therefore, does not transmit the death signal. Thus, these receptors represent a novel way of regulating cell sensitivity to a pro-apoptotic cytokine at the cell surface. TRAIL is expressed predominantly in spleen, lung, and prostate but also in many other tissues.Synonyms: APO2L, Apo-2 ligand, Apo-2L, TNF-related apoptosis-inducing ligand, TNFSF10, Tumor necrosis factor ligand superfamily member 10

ID gène: 8743

NCBI Accession: NP_003801

UniProt: P50591

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity