VEGFA
Reactivité: Humain
WB, ELISA, Neut
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: To detect hVEGF by direct ELISA (using 100 ml/well antibody solution) this antibodycan be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hVEGF. Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 mg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Antigène
VEGFA
(Vascular Endothelial Growth Factor A (VEGFA))