CXCL2 anticorps

N° du produit ABIN116268

Détail du produit anti-CXCL2 anticorps

Antigène: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CXCL2 est non-conjugé

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA), Neutralization (Neut)

Specificité: This antibody detects Mouse MIP-2. Other species not tested.

Purification: Affinity Chromatography employing immobilized murine mMIP-2 matrix

Immunogène: Highly pure (> 98%) E.coli derived recombinant Murine MIP-2

Information d'application

Indications d'application: Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of mMIP-2 (15.00 ng/mL), a concentration of 0.52-0.90 μg/mL of this antibody is required. ELISA: Indirect: Using 100 μL/well antibody solution, a concentration of 0.5 - 2.0 μg/mL isrequired. In conjunction with compatible secondary reagents, it allows the detection of atleast 0.2 - 0.4 ng/well of recombinant mMIP-2. Sandwich: Using 100 μL/well antibody solution, a concentration of 0.5 - 2.0 μg/mL isrequired. In conjunction with Biotinylated Anti-Murine MIP-2 (PP1082B) as a detectionantibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-2. Western Blot: 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents thedetection limit for recombinant mMIP-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.

Détails sur CXCL2

Antigène: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Autre désignation: CXCL2 / MIP2A (CXCL2 Produits)

Synonymes: anticorps CINC-2a, anticorps GRO2, anticorps GROb, anticorps MGSA-b, anticorps MIP-2a, anticorps MIP2, anticorps MIP2A, anticorps SCYB2, anticorps GRO3, anticorps Gro2, anticorps MIP-2, anticorps Mgsa-b, anticorps Mip2, anticorps Scyb, anticorps Scyb2, anticorps Mip-2, anticorps GROB, anticorps MIP-2alpha, anticorps C-X-C motif chemokine ligand 2, anticorps chemokine (C-X-C motif) ligand 2, anticorps CXCL2, anticorps Cxcl2

Sujet: GRO beta is a member of the CXC, or chemokine class. It contains the ELR domain immediately preceding the first cysteine residue near the amino terminus. Other chemokines in this group include IL8, GRO alpha/beta/gamma, mouse KC, ENA78, GCP2, PBP/CTAPIII/beta TG/NAP2. These chemokines act primarily on neutrophils as chemoattractants and activators, including neutrophil degradation with release of myloperoxidase and other enzymes. GRO beta was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. GRO beta is an approximately 8 kDa polypeptide of 73 amino acids. The precursor form of GRO beta consists of 100 amino acids. To generate the mature GRO beta, the precursor cleaves its amino terminal 27 amino acids. GRO beta shows 60 % amino acid homology to human GRO alpha and GRO gamma.Synonyms: C-X-C motif chemokine 2, GRO2, GROB, Gro-beta, Growth-regulated protein beta, MIP-2A, MIP2-alpha, Macrophage inflammatory protein 2-alpha, SCYB2

ID gène: 20310

NCBI Accession: NP_033166

UniProt: P10889

Pathways: Cellular Response to Molecule of Bacterial Origin