RANKL anticorps

N° du produit ABIN116282

Détail du produit anti-RANKL anticorps

Antigène: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RANKL est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificité: This antibody reacts with soluble RANK Ligand.

Purification: Affinity Chromatography.

Immunogène: Highly pure (>98%) recombinant Human sRANKL.

Information d'application

Indications d'application: Neutralization. Immunohistochemistry on Formalin-Fixed, Parrafin-embedded Sections: 0.25 μg/mL withan overnight incubation at 4 °C. Staining of Human metastatic carcinoma of lymph nodefrom breast tissue. An HRP-labeled polymer detection system was used with DABchromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer isrecommended. Sandwich ELISA: To detect Human sRANKL by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of at least 0.5-2.0 μg/mL of this antibody is required. This antigenaffinity purified antibody, in conjunction with Biotinylated anti-Human sRANKL (PP1086B1or PP1086B2) as a Detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human sRANKL. Western blot: To detect Human sRANKL by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur RANKL

Antigène: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Autre désignation: CD254 / RANKL (TNFSF11 Produits)

Synonymes: anticorps CD254, anticorps ODF, anticorps OPGL, anticorps OPTB2, anticorps RANKL, anticorps TRANCE, anticorps hRANKL2, anticorps sOdf, anticorps TNFSF11, anticorps Ly109l, anticorps OPG, anticorps Trance, anticorps cd254, anticorps odf, anticorps opgl, anticorps optb2, anticorps rankl, anticorps sodf, anticorps tnfsf11, anticorps trance, anticorps TNF superfamily member 11, anticorps tumor necrosis factor (ligand) superfamily, member 11, anticorps TNF receptor superfamily member 11b, anticorps TNF superfamily member 11 L homeolog, anticorps TNFSF11, anticorps Tnfsf11, anticorps TNFRSF11B, anticorps tnfsf11.L

Sujet: RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. There are three isoforms of RANKL. Human RANKL is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues). This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated that this protein may have a role in the regulation of cell apoptosis. RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.Synonyms: ODF, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, RANK Ligand, RANKL, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, TNFSF11, TRANCE, Tumor necrosis factor ligand superfamily member 11

ID gène: 8600

NCBI Accession: NP_003692

UniProt: O14788

Pathways: Signalisation NF-kappaB