VEGFA anticorps

N° du produit ABIN116334

Cet anticorps anti-VEGFA Polyclonal Lapin (ABIN116334) détecte spécifiquement VEGFA dans WB, IHC (p), EIA et Func. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour VEGFA anticorps (ABIN116334)

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VEGFA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Détail du produit anti-VEGFA anticorps

Specificité: Reacts with Vascular Endothelial Growth Factor (VEGF).

Purification: Affinity Chromatography.

Immunogène: E.coli derived highly pure (> 98%) recombinant Murine VEGF

Information d'application

Indications d'application: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of mVEGF (50.00 ng/mL), a concentration of 1.0-2.0 μg/mL of this antibody is required. ELISAIndirect: To detect mVEGF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well ofrecombinant mVEGF. Sandwich: To detect mVEGF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith biotinylated Anti-Murine VEGF (PP1113B) as a detection antibody, allows the detectionof at least 0.2-0.4 ng/well of recombinant mVEGF. Western BlotTo detect mVEGF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2-8 °C for one month or at -20 °C for longer.

Détails sur VEGFA

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Autre désignation: VEGF-A

Sujet: VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor

ID gène: 22339

NCBI Accession: NP_001020421

UniProt: Q00731

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling