NFKBIB anticorps (C-Term)

N° du produit ABIN116525

L’anticorps Lapin Polyclonal anti-NFKBIB a été validé pour WB, IP et EIA. Il convient pour détecter NFKBIB dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour NFKBIB anticorps (C-Term) (ABIN116525)

Antigène: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NFKBIB est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Détail du produit anti-NFKBIB anticorps

Épitope: C-Term

Specificité: Recognition is at the highly conserved C-terminal region. Anti-IκBβ may react non-specifically with other proteins. Control peptide (R1014CP) will compete only with the specific reaction of antiserum with IκBβ.

Purification: Prepared from monospecific antiserum by delipidation and defibrination

Immunogène: IkB beta peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Information d'application

Indications d'application: Suitable for Immunoprecipitation, Western blotting (171,000, shows a 45 kD band), ELISAand Dot Blot. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against IκBβ at a dilution of 1: 1000 followed by reaction with Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-IκBβ is suitable for thedetection by immunoblot of human, mouse and rat IκBβ.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 90 mg/mL (by Refractometry)

Conseil sur la manipulation: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.

Détails sur NFKBIB

Antigène: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Autre désignation: NFKBIB / IKBB

Sujet: NFκB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFκB bound to IκB. NFκB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFκB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IκB-α. IκB-α binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.Synonyms: I-kappa-B-beta, IkB-B, IkB-beta, IkappaBbeta, NF-kappa-B inhibitor beta, TR-interacting protein 9, TRIP9, Thyroid receptor-interacting protein 9

ID gène: 4793

NCBI Accession: NP_001001716

UniProt: Q15653

Pathways: Signalisation NF-kappaB, Activation of Innate immune Response, Maintenance of Protein Location, Toll-Like Receptors Cascades