MDH1 anticorps

N° du produit ABIN116777

Détail du produit anti-MDH1 anticorps

Antigène: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Reactivité: Porc

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp MDH1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other tissues and species may occur but have not been specifically determined.

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogène: Malate Dehydrogenase [Pig Heart].

Information d'application

Indications d'application: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of MalateDehydrogenase [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Sheep IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 12,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 80.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Détails sur MDH1

Antigène: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Autre désignation: MDHA (MDH1 Produits)

Synonymes: anticorps MDH-s, anticorps MDHA, anticorps MGC:1375, anticorps MOR2, anticorps ODS1, anticorps B230377B03Rik, anticorps D17921, anticorps Mor-2, anticorps Mor2, anticorps MDL1, anticorps Mdhl, anticorps MDH, anticorps mdh-s, anticorps mdha, anticorps mor2, anticorps MDH-1, anticorps Mdh, anticorps Mdh-1, anticorps Mdh2, anticorps MdhD, anticorps cMdh, anticorps sMdh, anticorps malate dehydrogenase 1, anticorps malic enzyme 2, anticorps malate dehydrogenase 1, NAD (soluble), anticorps malate dehydrogenase 1 S homeolog, anticorps malate dehydrogenase, cytoplasmic, anticorps Malate dehydrogenase 1, anticorps MDH1, anticorps ME2, anticorps Mdh1, anticorps mdh1.S, anticorps LOC100280767

Sujet: Malate dehydrogenase catalyzes the interconversion of L-malate and oxaloacetate using nicotinamide adenine dinucleotide (NAD) as a coenzyme. Malate dehydrogenase is found in all eukaryotic cells as two isozymes: mitochondrial (m-MDH) and cytoplasmic (soluble, s-MDH). Prokaryotes contain only a single form. The two isozymes, both consisting of two very similar subunits of about 35kD and having similar enzymatic activity appear as different proteins. There is structural similarity of mitochondrial malate dehydrogenase to L-3-hydroxyacyl CoA dehydrogenase and the cytoplasmic malate dehydrogenase to lactate dehydrogenase.Synonyms: MDH1, Malate dehydrogenase, cytoplasmic

ID gène: 396894

NCBI Accession: NP_999039

UniProt: P11708