ASK1 anticorps (pSer83)

N° du produit ABIN116930

L’anticorps Lapin Polyclonal anti-ASK1 a été validé pour WB. Il convient pour détecter ASK1 dans des échantillons de Humain.

Aperçu rapide pour ASK1 anticorps (pSer83) (ABIN116930)

Antigène: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ASK1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-ASK1 anticorps

Épitope: AA 76-87, pSer83

Specificité: This product is an IgG fraction antibody purified from antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed with ASK-1 from mouse sources. Reactivity with the kinase from other sources has not been determined.

Purification: Multi-step process.

Immunogène: This purified antibody was prepared from rabbit serum after repeated immunizations with a KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.

Information d'application

Indications d'application: This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimalreactivity by Western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, Immunohistochemistry andImmunoprecipitation. Recommended Dilutions: For immunoblotting a 1/1,000 dilution is recommended. A 155 kDa band corresponding to human ASK-1 is detected. Whole cell lysates from SW1353 canbe used as a positive control. For ELISA a 1/5,000-1/10,000 dilution is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 2.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use . Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur ASK1

Antigène: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Autre désignation: MEKK5 / ASK1

Sujet: ASK-1 (apoptosis signal-regulating kinase 1- also referred to as MEK Kinase-5 or MAPKKK5) is a novel serine/threonine MAP kinase kinase kinase (MAPKKK) component of the mitogen -activated protein (MAP) cascade that is activated in response to extracellular stimuli by cytokines, growth factors and environmental stresses and other factors. Overexpression of ASK-1 induces apoptotic cell death. ASK-1 is expressed in a variety of human and mouse tissues. The overall amino acid sequence identity between the mouse and human ASK1 is 91.9 % . ASK-1 interacts with CDKN1A (also known as p21, WAF1, CIP1). Please refer to the reference list at the end of this document for further information.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5

ID gène: 4217

NCBI Accession: NP_005914

UniProt: Q99683

Pathways: Signalisation MAPK, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response