This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody is primarily directed against 60,000 MW human PDK-1 and is useful in determining its presence in various assays.
Purification
Immunoaffinity chromatography.
Immunogène
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 301-312 of PDK-1 (genome data base accession number AC005591).
PDPK1
Reactivité: Humain, Souris, Rat
WB, ELISA, IHC, IF, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
This affinity purified antibody detects human PDK-1 and has been tested for use in ELISAand immunoblotting. Although untested, this reagent may be useful for Immunohistochemistry andImmunoprecipitation. It recognizes 60,000 MW human PDK-1. Reactivity in otherimmunoassays is unknown. Recommended Dilutions: This product has been assayed by immunoblot using HRPGoat-anti-Rabbit IgG [H&L] and Enhanced Chemiluminescence for detection. A workingdilution range of 1: 750 is suggested to detect PDK-1 in lysates derived from Sf9 insect cellsinfected with virus expressing myc-tagged human PDK1. In this experiment, approx. 10 μg of bothan untransfected sf9 cell lysate (not shown) and PDK-1 virus-infected Sf9 lysate wereseparated on a 10 % gel by SDS-PAGE followed by transfer to nitrocellulose. Primaryantibody reaction proceeded overnight at 4C using a 1: 750 dilution of the antibody in 1 %non-fat milk. This antibody predominantly recognizes a 60 kDa band corresponding tohuman PDK-1.
Restrictions
For Research Use only
Concentration
1.3 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the antibody (in aliquots) at -20 °C.
Antigène
PDPK1
(3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
anticorps pdpk1, anticorps MGC82080, anticorps PDK1, anticorps PDPK2, anticorps PRO0461, anticorps Pdk1, anticorps zgc:153787, anticorps 3'-phosphoinositide-dependent protein kinase 1, anticorps 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1, anticorps ATPDK1, anticorps AtPDK1, anticorps T32M21.110, anticorps T32M21_110, anticorps zgc:77318, anticorps 3-phosphoinositide dependent protein kinase 1, anticorps 3-phosphoinositide dependent protein kinase 1 L homeolog, anticorps 3-phosphoinositide dependent protein kinase-1, anticorps 3-phosphoinositide dependent protein kinase 1a, anticorps 3-phosphoinositide-dependent protein kinase 1, anticorps 3'-phosphoinositide-dependent protein kinase 1, anticorps 3-phosphoinositide dependent protein kinase 1b, anticorps PDPK1, anticorps pdpk1.L, anticorps pdpk1, anticorps Pdpk1, anticorps pdpk1a, anticorps LOC100380750, anticorps pdk-1, anticorps PDK1, anticorps pdpk1b
Sujet
PDK-1 (3-Phosphoinositide-Dependant Protein Kinase-1) phosphorylates AGC kinases. PDK-1 activates conventional PKC and PKCz (zeta) through phosphorylation of critical threonine residues in the activation loop. PDK-1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase-3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.Synonyms: 3-phosphoinositide-dependent proteinkinase 1, PDK-1, PDK1, PDPK1, hPDK1