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EGFR2 anticorps (AA 376-578) (FITC)

EGFR2 Reactivité: Humain IHC, WB, IF, ICC Hôte: Lapin Polyclonal FITC
N° du produit ABIN1175126
  • Antigène Voir toutes EGFR2 Anticorps
    EGFR2 (Epidermal Growth Factor Receptor 2 (EGFR2))
    Épitope
    AA 376-578
    Reactivité
    • 4
    • 3
    Humain
    Hôte
    • 6
    • 1
    Lapin
    Clonalité
    • 6
    • 1
    Polyclonal
    Conjugué
    • 4
    • 2
    • 1
    Cet anticorp EGFR2 est conjugé à/à la FITC
    Application
    • 7
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Séquence
    MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS- FLPES FDGDPASNTA PLQPEQLQVF ETLEEITGYL YISAWPDSLP DLSVFQNLQV IRGRILHNGA YSLTLQGLGI SWLGLRSLRE LGSGLALIHH NTHLCFVHTV PWDQLFRNPH QALLHTANRP EDECVGEGLA CHQLCARGHC WGPGPTQCVN CSQFLRGQEC VEECRVLQGL PREYVNARHC LPCHPECQPQ NGSVTCFG
    Specificité
    It has been selected for its ability to recognize EGFR2 in immunohistochemical staining and Western blotting.
    Purification
    Affinity Chromatography
    Immunogène
    Recombinant EGFR2 expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against EGFR2 conjugated to fitc.
    Isotype
    IgG
  • Indications d'application
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    Commentaires

    Content: The quality control contains recombinant EGFR2 (Phe376~Gly578) disposed in loading buffer.
    Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
    Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
    Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Conseil sur la manipulation
    Avoid repeated freeze/thaw cycles
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    Date de péremption
    12 months
  • Antigène
    EGFR2 (Epidermal Growth Factor Receptor 2 (EGFR2))
    Abstract
    EGFR2 Produits
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