PDPK1 anticorps (pSer241) (PE)

N° du produit ABIN1177136

Il existe 1 publication pour ce produit. L’anticorps anti-PDPK1 Monoclonal Souris est utilisé pour la détection de PDPK1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour ICS.

Aperçu rapide pour PDPK1 anticorps (pSer241) (PE) (ABIN1177136)

Antigène: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDPK1 est conjugé à/à la PE

Application: Intracellular Staining (ICS)

Clone: J66-653-44-17

Détail du produit anti-PDPK1 anticorps (PE)

Épitope: pSer241

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human PDPK1 Peptide

Isotype: IgG1 kappa

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-PDPK1 (PE) (ABIN1177136)

Komander, Kular, Deak, Alessi, van Aalten: "Role of T-loop phosphorylation in PDK1 activation, stability, and substrate binding." dans: The Journal of biological chemistry, Vol. 280, Issue 19, pp. 18797-802, (2005) (PubMed).

Détails sur PDPK1

Antigène: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Autre désignation: PDPK1

Sujet: The serine/threonine kinase 3-Phosphoinositide-Dependent Protein Kinase-1 (PDPK1, also known as PDK1) contributes to the activation of many important kinases in the insulin and IGF-1 signaling pathways. It acts downstream of phosphatidylinositol 3-kinase (PI3-kinase) to phosphorylate residues in the activation loops of many cellular kinases, including protein kinase B (PKB/Akt), PKC isoforms, p70 S6 kinase, and PDPK1 itself. The autophosphorylation of PDPK1 at serine 241 (S241) has recently been suggested to play a role in the regulation of PDPK1. It has been proposed that PDPK1 activity plays a key role in the regulation of various cellular events such as cell proliferation, differentiation, and apoptosis. The J666-653.44.17 monoclonal antibody recognizes the phosphorylated S241 in the activation loop of human PDPK1. The orthologous phosphorylation site in mouse and rat PDPK1 is S244.
Synonyms: PDK1 (pS241), PKB Kinase (pS241)

Pathways: Signalisation PI3K-Akt, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals