Phospholipase C gamma 2 anticorps (pTyr759) (Alexa Fluor 647)

N° du produit ABIN1177150

Détail du produit anti-Phospholipase C gamma 2 anticorps (Alexa Fluor 647)

Antigène: Phospholipase C gamma 2 (PLCG2)

Épitope: pTyr759

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Phospholipase C gamma 2 est conjugé à/à la Alexa Fluor 647

Application: Intracellular Staining (ICS)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human PLC-gamma2 Peptide

Clone: K86-689-37

Isotype: IgG1 kappa

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Références pour anticorps anti-Phospholipase C gamma 2 (Alexa Fluor 647) (ABIN1177150)

Holodick, Tumang, Rothstein: "Continual signaling is responsible for constitutive ERK phosphorylation in B-1a cells." dans: Molecular immunology, Vol. 46, Issue 15, pp. 3029-36, (2009) (PubMed).

Détails sur Phospholipase C gamma 2

Antigène: Phospholipase C gamma 2 (PLCG2)

Autre désignation: PLC-gamma 2 (PLCG2 Produits)

Sujet: The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLC-gamma is the only member that contains SH2 and SH3 domains. These domains enable it to interact with protein tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: PLC-g1, which is ubiquitously expressed, and PLC-g2, which is found primarily in the hematopoietic system. PLC-g2-null mice exhibit impaired B lymphocyte maturation and defects in Fc receptor functions. Phosphorylation of PLC-g2 at tyrosines 753 and 759 (Y759) is required for activation of PLC-g2 enzyme activity. PLC-g2 phosphorylation at Y759 can be induced by stimulation of the B cell receptor in Ramos cells, the collagen receptor in platelets, and the T cell receptor in Jurkat cells, and it occurs downstream of Btk and BLNK in the signaling cascade of activated B cells. The K86-689.37.73 antibody recognizes PLC-g2 phosphorylated at Y759 in the SH2-SH3 linker region.

Pathways: Signalisation RTK, Signalisation WNT, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling