Phospholipase C gamma 2 anticorps (PLCG2) (pTyr759) (Alexa Fluor 647)

Details for Product anti-PLCG2 Antibody No. ABIN1177150
Antigène
  • PLCgamma2
  • Plcg-2
  • APLAID
  • FCAS3
  • phospholipase C, gamma 2
  • phospholipase C gamma 2
  • phospholipase C, gamma 2 (phosphatidylinositol-specific)
  • Plcg2
  • PLCG2
Épitope
pTyr759
57
40
24
18
15
8
5
5
5
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
291
217
207
12
4
4
4
4
3
1
Hôte
Souris
279
12
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp Phospholipase C gamma 2 est conjugé à/à la Alexa Fluor 647
10
10
10
10
10
10
10
4
3
3
3
3
3
3
1
1
Application
Intracellular Staining (ICS)
170
104
54
40
38
38
14
12
11
8
2
1
Options
Marque BD Phosflow™
Immunogène Phosphorylated Human PLC-gamma2 Peptide
Clone K86-689-37
Isotype IgG1, kappa
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmids, Primers & others Phospholipase C gamma 2 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation PLC-gamma 2 (PLCG2 Antibody Extrait)
Sujet The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLC-gamma is the only member that contains SH2 and SH3 domains. These domains enable it to interact with protein tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: PLC-g1, which is ubiquitously expressed, and PLC-g2, which is found primarily in the hematopoietic system. PLC-g2-null mice exhibit impaired B lymphocyte maturation and defects in Fc receptor functions. Phosphorylation of PLC-g2 at tyrosines 753 and 759 (Y759) is required for activation of PLC-g2 enzyme activity. PLC-g2 phosphorylation at Y759 can be induced by stimulation of the B cell receptor in Ramos cells, the collagen receptor in platelets, and the T cell receptor in Jurkat cells, and it occurs downstream of Btk and BLNK in the signaling cascade of activated B cells. The K86-689.37.73 antibody recognizes PLC-g2 phosphorylated at Y759 in the SH2-SH3 linker region.
Pathways Signalisation RTK, Signalisation WNT, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling
Volume d'échantillon 20 μL
Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Stockage commentaire Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Produit citée dans: Holodick, Tumang, Rothstein: "Continual signaling is responsible for constitutive ERK phosphorylation in B-1a cells." dans: Molecular immunology, Vol. 46, Issue 15, pp. 3029-36, 2009 (PubMed).

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