PTK2B anticorps (pTyr402) (PE)

N° du produit ABIN1177156

Cet anticorps Souris Monoclonal détecte spécifiquement PTK2B dans ICS. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 6+ publications.

Aperçu rapide pour PTK2B anticorps (pTyr402) (PE) (ABIN1177156)

Antigène: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PTK2B est conjugé à/à la PE

Application: Intracellular Staining (ICS)

Clone: L68-1256-272

Détail du produit anti-PTK2B anticorps (PE)

Épitope: pTyr402

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human Pyk2 Peptide

Isotype: IgG2b kappa

Information d'application

Indications d'application: Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.

Commentaires:

BALB/c

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-PTK2B (PE) (ABIN1177156)

Anand, Cucchiarini, Terwilliger, Ganju: "The tyrosine kinase Pyk2 mediates lipopolysaccharide-induced IL-8 expression in human endothelial cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 8, pp. 5636-44, (2008) (PubMed).

Faure, Corvol, Toutant, Valjent, Hvalby, Jensen, El Messari, Corsi, Kadaré, Girault: "Calcineurin is essential for depolarization-induced nuclear translocation and tyrosine phosphorylation of PYK2 in neurons." dans: Journal of cell science, Vol. 120, Issue Pt 17, pp. 3034-44, (2007) (PubMed).

Orr, Murphy-Ullrich: "Regulation of endothelial cell function BY FAK and PYK2." dans: Frontiers in bioscience : a journal and virtual library, Vol. 9, pp. 1254-66, (2004) (PubMed).

Park, Avraham, Avraham: "RAFTK/Pyk2 activation is mediated by trans-acting autophosphorylation in a Src-independent manner." dans: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33315-22, (2004) (PubMed).

Gluck: "Acid sensing in renal epithelial cells." dans: The Journal of clinical investigation, Vol. 114, Issue 12, pp. 1696-9, (2004) (PubMed).

Avraham, Park, Schinkmann, Avraham: "RAFTK/Pyk2-mediated cellular signalling." dans: Cellular signalling, Vol. 12, Issue 3, pp. 123-33, (2000) (PubMed).

Détails sur PTK2B

Antigène: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Autre désignation: Pyk2

Sujet: Proline-rich tyrosine kinase 2 (Pyk2) is a member of the FAK subfamily of tyrosine protein kinases. Pyk2 responds to a wide variety of inflammatory and stress stimuli, such as cytokines, ligation of integrins or antigen receptors, mechanical strain, and cellular depolarization. A common characteristic of most Pyk2 stimuli is an increase in cytosolic free calcium. Pyk2 is involved in signaling pathways that regulate vital cellular functions in hematopoietic, epithelial, neuronal, endothelial, and other cell types. Activation of Pyk2 initiates its autophosphorylation at tyrosine 402 (Y402), followed by Src-mediated phosphorylation of other Pyk2 tyrosine sites, which enhances its activity and leads to downstream signal transduction through the MAPK and JNK pathways. The L68-1256.272 monoclonal antibody recognizes the phosphorylated Y402 of activated Pyk2.
Synonyms: Protein tyrosine kinase 2beta, FAK2, FADK 2, CAKbeta, CADTK, RAFTK

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling