Bcl-2 anticorps (pSer70)

N° du produit ABIN1177235

L’anticorps Souris Monoclonal anti-Bcl-2 a été validé pour WB, IHC (f) et ICS. Il convient pour détecter Bcl-2 dans des échantillons de Humain. Il y a 5+ publications disponibles.

Aperçu rapide pour Bcl-2 anticorps (pSer70) (ABIN1177235)

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Bcl-2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS)

Clone: N46-467

Détail du produit anti-Bcl-2 anticorps

Épitope: pSer70

Marque: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Peptide

Isotype: IgG1

Information d'application

Indications d'application: IHC(F): Antigen retrieval required

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Bcl-2 (ABIN1177235)

Geng, Tang, Li, Yang, Choi, Kazim, Zhang: "Allyl isothiocyanate arrests cancer cells in mitosis, and mitotic arrest in turn leads to apoptosis via Bcl-2 protein phosphorylation." dans: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 32259-67, (2011) (PubMed).

Sarkar, Korolchuk, Renna, Imarisio, Fleming, Williams, Garcia-Arencibia, Rose, Luo, Underwood, Kroemer, OKane, Rubinsztein: "Complex inhibitory effects of nitric oxide on autophagy." dans: Molecular cell, Vol. 43, Issue 1, pp. 19-32, (2011) (PubMed).

Pattingre, Bauvy, Carpentier, Levade, Levine, Codogno: "Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy." dans: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 2719-28, (2009) (PubMed).

Deng, Xiao, Lang, Gao, Ruvolo, May: "Novel role for JNK as a stress-activated Bcl2 kinase." dans: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23681-8, (2001) (PubMed).

Ling, Tornos, Perez-Soler: "Phosphorylation of Bcl-2 is a marker of M phase events and not a determinant of apoptosis." dans: The Journal of biological chemistry, Vol. 273, Issue 30, pp. 18984-91, (1998) (PubMed).

Détails sur Bcl-2

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Autre désignation: Bcl-2

Sujet: The clone N46-467 monoclonal antibody specifically binds to Bcl-2 (pS70), ie, the Bcl-2 protein phosphorylated at the Ser70 site. Bcl-2 is a ~ 26 kDa intracellular, integral membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane. Bcl-2 is encoded by the BCL2 (B-cell CLL/lymphoma 2) gene and is also known as Apoptosis regulator Bcl-2. Members of the Bcl-2 family play a major role in regulating the response of cells to apoptotic signals. Bcl-2 is one of the anti-apoptotic members of the Bcl-2 family. Bcl-2 knockout mice showed pronounced lymphoid apoptosis and other apoptosis related lesions later in life. Bcl-2 is a proto-oncogene because it blocks apoptosis and provides a selective survival advantage in many cell types and thus contributes to tumorigenesis. It has been implicated in several types of cancers, such as breast, prostate, and melanoma . Bcl-2 contains multiple phosphorylation sites including Thr56, Ser70, Thr74 and Ser87. Phosphorylation of Bcl-2 Ser70 has been shown to be a mitotic marker. Phosphorylation at this site regulates Bcl-2's anti-apoptotic activity and has recently been implicated in promoting autophagy. Several studies have shown that Bcl-2 phosphorylation is caused by c-Jun N-terminal kinase (JNK).
Synonyms: BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, PPP1R50

Poids moléculaire: ~28 kDa

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling