CD93 anticorps

N° du produit ABIN1177363

Détail du produit anti-CD93 anticorps

Antigène: CD93

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD93 est non-conjugé

Application: Flow Cytometry (FACS), Neutralization (Neut)

Marque: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Clone: R139

Isotype: IgG2b kappa

Information d'application

Indications d'application: This NA/LE format is useful for in vitro functional studies. This antibody has been tested for LAL assay for low endotoxin level and by immunofluorescent staining in flow cytometric analysis to assure specificity and reactivity. Immunofluorescent Staining and Flow Cytometric Analysis: The staining technique and controls are described in detail by C. Prussin and D. Metcalfe. A suitable mouse IgG2b isotype control for assessing the level of background staining on human cells is recommended: use at comparable concentrations to antibody of interest (e.g., ≤ 0.125 µg mAb/1 million cells).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Références pour anticorps anti-CD93 (ABIN1177363)

Danet, Luongo, Butler, Lu, Tenner, Simon, Bonnet: "C1qRp defines a new human stem cell population with hematopoietic and hepatic potential." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 16, pp. 10441-5, (2002) (PubMed).

Nepomuceno, Ruiz, Park, Tenner: "C1qRP is a heavily O-glycosylated cell surface protein involved in the regulation of phagocytic activity." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 6, pp. 3583-9, (1999) (PubMed).

Tenner: "C1q receptors: regulating specific functions of phagocytic cells." dans: Immunobiology, Vol. 199, Issue 2, pp. 250-64, (1999) (PubMed).

Nepomuceno, Tenner: "C1qRP, the C1q receptor that enhances phagocytosis, is detected specifically in human cells of myeloid lineage, endothelial cells, and platelets." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 4, pp. 1929-35, (1998) (PubMed).

Nepomuceno, Henschen-Edman, Burgess, Tenner: "cDNA cloning and primary structure analysis of C1qR(P), the human C1q/MBL/SPA receptor that mediates enhanced phagocytosis in vitro." dans: Immunity, Vol. 6, Issue 2, pp. 119-29, (1997) (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." dans: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Guan, Robinson, Goodman, Tenner: "Cell-surface protein identified on phagocytic cells modulates the C1q-mediated enhancement of phagocytosis." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 8, pp. 4005-16, (1994) (PubMed).

Guan, Burgess, Robinson, Goodman, McTigue, Tenner: "Phagocytic cell molecules that bind the collagen-like region of C1q. Involvement in the C1q-mediated enhancement of phagocytosis." dans: The Journal of biological chemistry, Vol. 266, Issue 30, pp. 20345-55, (1991) (PubMed).

Détails sur CD93

Antigène: CD93

Autre désignation: CDw93 (CD93 Produits)

Synonymes: anticorps 6030404G09Rik, anticorps AA145088, anticorps AA4.1, anticorps AW555904, anticorps C1qr1, anticorps C1qrp, anticorps Ly68, anticorps C1QR1, anticorps C1qR(P), anticorps C1qRP, anticorps CDw93, anticorps ECSM3, anticorps MXRA4, anticorps dJ737E23.1, anticorps C1qRp, anticorps CD93 antigen, anticorps CD93 molecule, anticorps Cd93, anticorps CD93

Sujet: The immunogen used to raise R139 antibody was C1q-binding protein preparation derived from U937 cell lysates, as described. Human CDw93 (C1qRp) is a 631 AA, single chain type I membrane glycoprotein expressed on cells of myeloid origin, endothelial cells, and hematopoietic progenitor cells. Human, murine and rat protein sequences have been deduced from cDNA clones and are known to be similar in sequence and organization. CDw93 (C1qRp) binds C1q, the recognition subunit of the first component (C1) of the complement pathway, as well as MBL (Mannose-binding-lectin) and SPA (Pulmonary Surfactant Protein A). Multivalent interaction of CDw93 (C1qRp) expressing cells with C1q, MBL, and SPA, induces enhancement of phagocytosis of suboptimally opsonized particles and/or cellular debris. Antibody R139 neutralizes/blocks C1q-mediated enhancement of phagocytosis, as reported. In addition clone R139 is suitable to detect CDw93 (C1qRp) expression on cells by flow cytometry, CDw93 (C1qRp) in cellular lysates by Western blotting or immunoprecipitation. CDw93 (C1qRp) has been reported to define a human stem cell population with hematopoietic and hepatic potential.
Synonyms: C1qRp