CD8B anticorps

N° du produit ABIN118499

L’anticorps Souris Monoclonal anti-CD8B a été validé pour FACS, WB et IP. Il convient pour détecter CD8B dans des échantillons de Humain et Singe. Il y a 1 publication disponible.

Aperçu rapide pour CD8B anticorps (ABIN118499)

Antigène: CD8B (CD8b Molecule (CD8B))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8B est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Clone: 2ST8-5H7

Détail du produit anti-CD8B anticorps

Specificité: This antibody recognises the CD8 cell surface antigen. The antibody binds to the beta chain, recognising an epitope that is dependent upon expression of both CD8 alpha and CD8 beta. Reacts with Cynomolgus Monkey.

Purification: Purified

Immunogène: Normal Human T lymphocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-CD8B (ABIN118499)

Heigele, Schindler, Gnanadurai, Leonard, Collins, Kirchhoff: "Down-modulation of CD8αβ is a fundamental activity of primate lentiviral Nef proteins." dans: Journal of virology, Vol. 86, Issue 1, pp. 36-48, (2012) (PubMed).

Détails sur CD8B

Antigène: CD8B (CD8b Molecule (CD8B))

Autre désignation: CD8 beta

Sujet: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain

ID gène: 926

NCBI Accession: NP_001171571

UniProt: P10966

Pathways: TCR Signaling