CD11b anticorps (PE)

N° du produit ABIN120415

Cet anticorps Souris Monoclonal détecte spécifiquement CD11b dans FACS. Il présente une réactivité envers Rat.

Aperçu rapide pour CD11b anticorps (PE) (ABIN120415)

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11b est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Clone: OX-42

Détail du produit anti-CD11b anticorps (PE)

Purification: Ion exchange chromatography

Immunogène: Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Clone OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Functionally, clone OX-42 inhibits complement mediated rosettes. We recommend the use of ABIN182004 for use in functional studies.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL distilled water.

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Original vial: Stable at 2-8 °C for 1 year from despatch. After reconstitution: Aliquot and freeze at -20 °C or lower. Do not freeze working dilutions!

Date de péremption: 12 months

Détails sur CD11b

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Autre désignation: CD11b

Sujet: CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8 % spleen cells, 44 % bone marrow cells and less than 1 % of thymocytes and is commonly used as a microglial marker in nervous tissue.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

ID gène: 10116

UniProt: Q63001

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation