CD28 anticorps (Biotin)

N° du produit ABIN120437

L’anticorps Souris Monoclonal anti-CD28 a été validé pour FACS. Il convient pour détecter CD28 dans des échantillons de Rat.

Aperçu rapide pour CD28 anticorps (Biotin) (ABIN120437)

Antigène: CD28

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD28 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Clone: JJ319

Détail du produit anti-CD28 anticorps (Biotin)

Specificité: Antibody SM268B recognizes a 90 kDa homeodimeric cell surface glycoprotein (CD28). Results of tissue Distribution by Flow Cytometry Analysis: Rat strain: Fischer Cell concentration: 1x10e6 cells per test Antibody concentration used: 1.0 μg/10e6 cells Isotypic control: Biotin Mouse IgG1 Cell source percentage of cells stained above control Thymus: 25.4 % Splenic T Cells*: 73.2 % *(T cells isolated with a Rat T Cell Recovery Column).

Purification: Protein G Chromatography

Immunogène: Rat CD28 transfected A20J cells

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry (for details please see Protocols below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This clone can costimulate T cell proliferation and IL-2 secretion by resting rat T cells.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Date de péremption: 6 months

Détails sur CD28

Antigène: CD28

Autre désignation: CD28

Sujet: CD28 has been found to be a potent costimulatory receptor on T cells. It is expressed on all peripheral rat alpha/beta and most gamma/delta T cells, as well as on approximately half of all NK cells.Synonyms: T-cell-specific surface glycoprotein CD28, TP44

ID gène: 25660

NCBI Accession: NP_037253

UniProt: P31042

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response