ITGB1 anticorps

N° du produit ABIN120575

L’anticorps Souris Monoclonal anti-ITGB1 a été validé pour WB, FACS et IP. Il convient pour détecter ITGB1 dans des échantillons de Humain, Porc et Chien.

Aperçu rapide pour ITGB1 anticorps (ABIN120575)

Antigène: ITGB1 (Integrin beta 1 (ITGB1))

Reactivité: Humain, Porc, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGB1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: MEM-101A

Détail du produit anti-ITGB1 anticorps

Réactivité croisée (Details): Species reactivity (tested):Human, Porcine and Canine (Dog)

Purification: Affinity Chromatography on Protein A

Immunogène: RAJI human Burkitt's lymphoma cell line

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: use 5 μL of neat antibody to label 10^6 cells. Immunoprecipitation. Western blotting: 2 mg/mL, Positive control: JURKAT human leukemia T-cell lysate Kg-1a human leukemia cell lysate Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS Application note: Non-reducing conditions. SDS-PAGE (6 % separating gel, 4 % stacking gel). Recommended secondary antibody - anti mouse IgG/HRP, dilution 1:2000, 60 min on vertical incubator.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM Sodium Azide as preservative, approx. pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur ITGB1

Antigène: ITGB1 (Integrin beta 1 (ITGB1))

Autre désignation: CD29 / Integrin beta-1

Sujet: CD29 (b1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 dibberent a chains (a1-a6, CD49a-f) determining the binding properties of b1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most b1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of b1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a b1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1b.Synonyms: FNRB, Fibronectin receptor subunit beta, ITGB1, Integrin VLA-4 subunit beta, MDF2, MSK12

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome