HIV Protease anticorps

N° du produit ABIN125709

Détail du produit

Antigène: HIV Protease

Reactivité: Human Immunodeficiency Virus (HIV)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB), Functional Studies (Func)

Specificité: The antibody 1696 recognizes free N-terminus of mature HIV protease (HIV-1 and HIV-2), an enzyme that hydrolyzes polyproteins of HIV viruses into functional proteins. The antibody 1696 does not react with the precursor.

Réactivité croisée (Details): HIV

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Bacterially expressed full-length HIV-1 protease

Clone: 1696

Isotype: IgG1

Information d'application

Indications d'application: Functional application: The antibody 1696 strongly inhibits the enzyme activity of HIV-1 and HIV-2 proteases.
Western blotting: Recommended dilution: 0.5 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Agent conservateur: Azide free

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Référence

Dunn, Boyer, McWilliams, Smith, Hughes: "Mutations in human immunodeficiency virus type 1 reverse transcriptase that make it sensitive to degradation by the viral protease in virions are selected against in patients." dans: Virology, Vol. 484, pp. 127-35, (2015) (PubMed).

Dunn, Boyer, Clark, Hughes: "Mutations in HIV-1 reverse transcriptase cause misfolding and miscleavage by the viral protease." dans: Virology, (2013) (PubMed).

Bartonová, Král, Sieglová, Brynda, Fábry, Horejsí, Kozísek, Sasková, Konvalinka, Sedlácek, Rezácová: "Potent inhibition of drug-resistant HIV protease variants by monoclonal antibodies." dans: Antiviral research, Vol. 78, Issue 3, pp. 275-7, (2008) (PubMed).

OLoughlin, Greene, Matsumura: "Diversification and specialization of HIV protease function during in vitro evolution." dans: Molecular biology and evolution, Vol. 23, Issue 4, pp. 764-72, (2006) (PubMed).

Lescar, Brynda, Rezacova, Stouracova, Riottot, Chitarra, Fabry, Horejsi, Sedlacek, Bentley: "Inhibition of the HIV-1 and HIV-2 proteases by a monoclonal antibody." dans: Protein science : a publication of the Protein Society, Vol. 8, Issue 12, pp. 2686-96, (2000) (PubMed).

Détail du antigène

Antigène: HIV Protease

Classe de substances: Viral Protein

Sujet: The HIV protease (PR) hydrolyzes polyproteins of HIV virus into functional protein products that are essential for its assembly and subsequent activity. This maturation process occurs as the virion buds from the host cell. HIV protease inhibitors are used in the treatment of patients with AIDS and were considered the first breakthrough in over a decade of AIDS research. HIV protease inhibitors can lower the viral load carried by AIDS patents.,HIV-1 PR, HIV-2 PR