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ZAP70 anticorps (C-Term)

ZAP70 Reactivité: Humain WB, FACS, ICC Hôte: Souris Monoclonal ZAP-03 unconjugated
N° du produit ABIN125756
  • Antigène Voir toutes ZAP70 Anticorps
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    Épitope
    • 41
    • 40
    • 26
    • 20
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    • 16
    • 13
    • 10
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    • 1
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    • 1
    • 1
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    C-Term
    Reactivité
    • 296
    • 129
    • 49
    • 8
    • 7
    • 7
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    Humain
    Hôte
    • 220
    • 72
    • 2
    • 2
    • 1
    Souris
    Clonalité
    • 198
    • 98
    Monoclonal
    Conjugué
    • 146
    • 18
    • 16
    • 16
    • 12
    • 8
    • 8
    • 8
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    • 7
    • 7
    • 7
    • 4
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    • 2
    • 2
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    • 2
    • 1
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    • 1
    Cet anticorp ZAP70 est non-conjugé
    Application
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    • 70
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    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Specificité
    The antibody ZAP-03 reacts with ZAP70, a 70 kDa protein tyrosine kinase expressed in T and NK cells (intracellular antigen). ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
    Réactivité croisée (Details)
    Human
    Purification
    Purified by protein-A affinity chromatography.
    Pureté
    > 95 % (by SDS-PAGE)
    Immunogène
    Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP70 with histidine tag
    Clone
    ZAP-03
    Isotype
    IgG1
  • Indications d'application
    Flow cytometry: Intracellular staining, recommended dilution: 2-5 μg/mL, positive control: HPB-ALL human peripheral blood T cell leukemia cell line.
    Western blotting: Recommended dilution: 0.5-2 μg/mL.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Do not freeze.
  • Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." dans: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).

  • Antigène
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    Autre désignation
    ZAP-70 (ZAP70 Produits)
    Synonymes
    anticorps SRK, anticorps STCD, anticorps STD, anticorps TZK, anticorps ZAP-70, anticorps 70kDa, anticorps AI327364, anticorps Srk, anticorps mrtle, anticorps mur, anticorps zap70, anticorps MGC80670, anticorps MGC88962, anticorps ZAP70, anticorps zgc:110383, anticorps zeta chain of T-cell receptor associated protein kinase 70, anticorps putative tyrosine-protein kinase zap-70, anticorps zeta-chain (TCR) associated protein kinase, anticorps zeta chain of T cell receptor associated protein kinase 70, anticorps zeta-chain (TCR) associated protein kinase 70kDa, anticorps zeta chain of T cell receptor associated protein kinase 70kDa L homeolog, anticorps zeta chain of T cell receptor associated protein kinase 70kDa, anticorps tyrosine-protein kinase ZAP-70, anticorps ZAP70, anticorps Smp_149470, anticorps Zap70, anticorps zap70.L, anticorps zap70, anticorps LOC707555, anticorps LOC100342021, anticorps LOC100390922, anticorps LOC100542877
    Sujet
    Zeta chain of T cell receptor associated protein k,The ZAP70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP70 gene mutations resulting in lower ZAP70 protein expression levels or expression of catalytically inactive ZAP70 proteins, have been identified. ZAP70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP70 expression on B cell was shown to be correlated with disease progression and survival. ZAP70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP70 on Tyr493 in the activation loop. ZAP70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.,ZAP-70, ADMIO2
    ID gène
    7535
    UniProt
    P43403
    Pathways
    TCR Signaling, Ubiquitin Proteasome Pathway
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