ZAP70 anticorps (AA 247-382)

N° du produit ABIN6940913

Cet anticorps anti-ZAP70 Monoclonal Souris (Clone ZAP70-2035) (ABIN6940913) détecte spécifiquement ZAP70 dans IHC, FACS, ELISA et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour ZAP70 anticorps (AA 247-382) (ABIN6940913)

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ZAP70 est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Clone: ZAP70-2035

Détail du produit anti-ZAP70 anticorps

Épitope: AA 247-382

Fonction: ZAP70 (Chronic Lymphocytic Leukemia Marker) Antibody

Specificité: ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells.Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25 % of chronic lymphocytic leukemia (CLL) cases as well.Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment of human ZAP70 protein (around aa 247-382) (exact sequence is proprietary)

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: Jurkat cells. Tonsil or lymph node.

Known Application: Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL),ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur ZAP70

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Autre désignation: ZAP70

Sujet:

Selective T cell defect, SRK, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70,TZK, Zeta chain associated protein kinase 70 kDa

Jurkat cells. Tonsil or lymph node.

Poids moléculaire: 70kDa

ID gène: 7535, 234569

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway