MAPK10 anticorps

N° du produit ABIN126824

L’anticorps anti-MAPK10 Monoclonal Souris est utilisé pour la détection de MAPK10 dans des échantillons de Humain. Il a été validé pour WB.

Aperçu rapide pour MAPK10 anticorps (ABIN126824)

Antigène: MAPK10 (Mitogen-Activated Protein Kinase 10 (MAPK10))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAPK10 est non-conjugé

Application: Western Blotting (WB)

Clone: 4G6

Détail du produit anti-MAPK10 anticorps

Specificité: Antibody AM00080PU-N specifically interacts with the N-terminus of SAPK1 beta/jnk3 kinase. Shows no cross-reactivity with jnk1 or jnk2

Attributs du produit: Synonyms: Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3, JNK3A, PRKM10, MAPkinase p49 3F12, Mitogen-activated protein kinase 10

Purification: Purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography

Ingrédients: incl. positive Control

Immunogène: Peptide conjugated to hemocyaninEpitope: N-terminusRemarks: Molecular weight of antigen 54 kDa

Isotype: IgG

Information d'application

Indications d'application: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use cell lysate from untreated SH-SY5Y cells delivered with this product as positive control: see protocol below!
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Positive Control Cell Lysate: Untreated SH-SY5YFormat: Lyophilized cell lysate from serum starved SH-SY5Y cells. Reconstitute by addition of 200 µl H20. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Applications: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -20 °C to -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at2-8 °C up to 3 months. Avoid repeated freeze / thaw cycles.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur MAPK10

Antigène: MAPK10 (Mitogen-Activated Protein Kinase 10 (MAPK10))

Autre désignation: MAPK10

Sujet: Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. The SAPK1/jnk family consists of 3 isoforms: SAPK1alpha/jnk2, SAPK1beta/jnk3, SAPK1gamma/jnk1.Synonyms: JNK3A, MAP kinase p49 3F12, Mitogen-activated protein kinase 10, PRKM10, Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3

ID gène: 5602

UniProt: P53779

Pathways: Signalisation MAPK, Signalisation WNT, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades