L’anticorps Souris Monoclonal anti-MAP2K7 a été validé pour WB, IF, IHC (fro) et EIA. Il convient pour détecter MAP2K7 dans des échantillons de Humain, Souris, Rat et Chien.
MAP2K7
Reactivité: Humain, Souris, Rat
WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated SW480 cells. ELISA: 0.1 μg/mL. Immunocytochemistry. Immunohistochemistry on frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1 mL H2O (15 min, RT).
Buffer
1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C/-80 °C
Stockage commentaire
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Shelf life: one year from despatch.
Date de péremption
12 months
Antigène
MAP2K7
(Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7))
Autre désignation
MAP2K7
Sujet
The stress response of mammalian cells results in activation of kinases of the SAPK1 and SAPK2 families. The SAP kinases are specifically activated by dual phosphorylation of a conserved T-X-Y motif by MAP kinase kinases (MKK). The 3 isoforms of the SAPK1 family (SAPK1α/jnk2, SAPK1β/jnk3, SAPK1γ/jnk1) are synergistically activated by phosphorylation of the threonine residue by MKK7 and the tyrosine residue by MKK4.Synonyms: Dual specificity mitogen-activated protein kinase kinase 7, JNK-activating kinase 2, MAP kinase kinase 7, MAPK/ERK kinase 7, MAPKK 7, MEK7, MKK7, NKK2, c-Jun N-terminal kinase kinase 2