Phosphotyrosine anticorps

N° du produit ABIN126879

Détail du produit anti-Phosphotyrosine anticorps

Antigène: Phosphotyrosine

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Polyclonal

Conjugué: Cet anticorp Phosphotyrosine est non-conjugé

Application: Western Blotting (WB)

Purification: Purified

Ingrédients: incl. positive Control

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: Positive Control for Immunonlot Applications. Phosphotyrosine Molecular Weight Marker:

Restrictions: For Research Use only

Stockage

Détails sur Phosphotyrosine

Antigène: Phosphotyrosine

Abstract: Phosphotyrosine Produits

Classe de substances: Amino Acid

Sujet: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the nucleus. Phosphorylated epitopes may serve as docking sites for the assembley of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosin kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via the interaction of phosphotyrosine residues with SH2 or PTB domains. Antibodies direct against phosphorylated epitopes recognize the phosphorylated amino acid in the context of the surrounding amino acid sequence. Recognition is therefore dependent on 2 criteria: 1) phosphorylation and 2) the surrounding amino acid motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Since the amino acid sequence varies between different phosphorylation sites, certain proteins - though phosphorylated - may not be detected by the antibody. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity.