Bcl-2 anticorps (N-Term)

N° du produit ABIN126981

Cet anticorps anti-Bcl-2 Polyclonal Lapin (ABIN126981) détecte spécifiquement Bcl-2 dans WB, IP et IHC (fro). L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Bcl-2 anticorps (N-Term) (ABIN126981)

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Bcl-2 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Bcl-2 anticorps

Épitope: N-Term

Specificité: Detects a 25 kDa protein, corresponding to the apparent molecular weight of the alpha isoform of Bcl-2 on SDS-PAGE immunoblots.

Purification: Affinity-purified

Immunogène: Synthetic peptide corresponding to the N-terminus of human Bcl-2

Information d'application

Indications d'application: Western blot analysis (0.5-4 μg/mL). Immunoprecipation (4-8 μg/mL). Immunohistochemistry on frozen sections (10-20 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid Repeated freeze/defrost cycles.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. For long-term storage, aliquot and freeze at -20 °C to -70 °C.

Détails sur Bcl-2

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Autre désignation: Bcl-2

Sujet: Bcl-2 and its homologs belong to a family of proteins that either promotes or prevent apoptosis. Bcl-2 alpha and beta are the alternatively spliced isoforms of 25 kDa and 22 kDa mitochondrial integral membrane proteins that inhibit apoptosis. Bcl-2 was identified as a gene translocated from chromosome 18 to the immunoglobulin (Ig) heavy chain locus on chromosome 14 in B lymphocytes, a condition that is associated with the onset of human follicular B cell lymphomas. This interchromosomal translocation leads to constitutive expression of Bcl-2, which results in increased survival and expansion of follicular center cell population.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha

ID gène: 596

NCBI Accession: NP_000624

UniProt: P10415

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling