SGK1 anticorps (AA 400-431)

N° du produit ABIN129525

N° du produit (Fournisseur): 200-401-844

Cet anticorps anti-SGK1 est un anticorps Lapin Polyclonal détectant SGK1 dans WB, ELISA et IHC. Adapté pour Humain.

Aperçu rapide pour SGK1 anticorps (AA 400-431) (ABIN129525)

Antigène: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SGK1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-SGK1 anticorps

Épitope: AA 400-431

N° du produit (Fournisseur): 200-401-844

Fournisseur: Rockland

Fonction: SKG-1 Antibody

Réactivité croisée (Details): Anti-SKG-1 Antibody is directed against human SGK-1 protein.

Attributs du produit: Synonyms: rabbit anti-SKG-1 Antibody, Serum/glucocorticoid-regulated kinase 1, SGK 1 antibody, SGK1 antibody, Cellular stress response antibodies, Serine/threonine-protein kinase Sgk1

Purification: The product was protein A purified from monospecific antiserum.

Stérilité: Sterile filtered

Immunogène:

Immunogen: SKG-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 400-431 of human SGK-1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 10 μg/mL to 20 μg/mL

Application Note: SKG-1 Antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~49 kDa in size corresponding to SGK-1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:4,000 - 1:16,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store SKG-1 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur SGK1

Antigène: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Autre désignation: SGK1

Sujet: Background: SGK-1 (also called Serine/threonine-protein kinase Sgk1 and Serum/glucocorticoid-regulated kinase 1) is a protein kinase that plays an important role in cellular stress response.  SGK1 activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. This kinase mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A and phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, Kv1.3, or EAAT1. SGK1 is localized to the cytoplasm and upon phosphorylation is translocated to the nucleus.  The kinase is expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung.  Induction occurs upon exposure to glucocorticoids and by excessive extracellular glucose or TGF-beta, in cultured cells.   SGK-1 is regulated by phosphorylation.  Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1. The kinase is ubiquitinated by NEDD4L, which promotes proteasomal degradation.

ID gène: 6446

UniProt: O00141

Pathways: Signalisation MAPK, Signalisation Notch, Steroid Hormone Mediated Signaling Pathway