CHEK2 anticorps (pThr68)

N° du produit ABIN129576

N° du produit (Fournisseur): 600-401-280

Cet anticorps anti-CHEK2 est un anticorps Lapin Polyclonal détectant CHEK2 dans WB, ELISA et IP. Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour CHEK2 anticorps (pThr68) (ABIN129576)

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHEK2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-CHEK2 anticorps

Épitope: pThr68

N° du produit (Fournisseur): 600-401-280

Fournisseur: Rockland

Fonction: CHK2 phospho T68 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against the phosphorylated form of human CHK2 at the pT68 residue.

Attributs du produit: Synonyms: rabbit anti-CHK2 pT68 Antibody, Serine/threonine-protein kinase Chk2, CHK-2, CHK 2, CDS1, RAD53, CHEK2, Checkpoint kinase 2, Hucds1

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 50-75 of Human CHK2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 61 kDa in size corresponding to CHK2 by western blotting in the appropriate cell lysate or extract.  Less than 1 % reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pT68 of CHK2.

Western Blot Dilution: 1:200 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:10,000 - 1:70,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-CHEK2 (ABIN129576)

Lu, Lou, Peng, Xie, Nghiem, Conney: "Effect of caffeine on the ATR/Chk1 pathway in the epidermis of UVB-irradiated mice." dans: Cancer research, Vol. 68, Issue 7, pp. 2523-9, (2008) (PubMed).

Détails sur CHEK2

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Autre désignation: CHEK2

Sujet: Background: CHK2 (also known as CHEK2, Protein kinase CHK2 isoform a, and checkpoint-like protein) is a serine/ threonine-protein kinase involved in the control of cell cycle checkpoints and may also participate in transduction of the DNA damage and replicational stress signals. CHK2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of CHK2 contains a series of seven serine and threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. Indeed, after DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR.  The SQ/TQ cluster domain, therefore, seems to have a regulatory function.  Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain.  CHK2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, preventing the entry into mitosis. This kinase may have a role in meiosis as well. Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block.

ID gène: 11200

NCBI Accession: NP_001005735

UniProt: O96017

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire