MDC1 anticorps (AA 679-694)

N° du produit ABIN129583

N° du produit (Fournisseur): 600-401-453

Cet anticorps Lapin Polyclonal détecte spécifiquement MDC1 dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour MDC1 anticorps (AA 679-694) (ABIN129583)

Antigène: MDC1 (Mdc1 (MDC1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MDC1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-MDC1 anticorps

Épitope: AA 679-694

N° du produit (Fournisseur): 600-401-453

Fournisseur: Rockland

Fonction: MDC1 Antibody

Attributs du produit: Synonyms: rabbit anti-MDC1 antibody, MDC-1, MDC 1 antibody, Mediation of DNA damage checkpoint 1 antibody, NFBD1, Nuclear factor with BRCT domains 1

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 679-694 of Human MDC1 (mediator of DNA damage checkpoint protein 1).   MDC1 is hyper-phosphorylated in an ATM-dependent manner.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide.  Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:500- 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur MDC1

Antigène: MDC1 (Mdc1 (MDC1))

Autre désignation: MDC1

Sujet: Background: Mediator of DNA damage checkpoint protein 1 (MDC1) is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. It may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also it is required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.

ID gène: 9656

NCBI Accession: NP_055456

UniProt: Q14676