WRNIP1 anticorps (Internal Region)

N° du produit ABIN129611

N° du produit (Fournisseur): 600-401-676

L’anticorps Lapin Polyclonal anti-WRNIP1 a été validé pour WB, ELISA et IHC. Il convient pour détecter WRNIP1 dans des échantillons de Humain.

Aperçu rapide pour WRNIP1 anticorps (Internal Region) (ABIN129611)

Antigène: WRNIP1 (Werner Helicase Interacting Protein 1 (WRNIP1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp WRNIP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-WRNIP1 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-676

Fournisseur: Rockland

Fonction: WHIP Antibody

Réactivité croisée (Details): Reactivity is expected against human, mouse, rat and monkey WHIP1 protein.

Attributs du produit: Synonyms: rabbit anti-WHIP antibody, ATPase WRNIP 1 antibody, ATPase WRNIP1 antibody, Werner helicasae interacting protein 1 antibody, WRNIP-1

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide immobilized to a solid phase.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the WHIP1 protein.  The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 kDa and 69.5 kDa, respectively.  The immunogen sequence also shows 100% homology to WHIP1 from mouse, rat and monkey sequences.  Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested by WB and ELISA. Anti-WHIP is useful in western blotting against HEK293 whole cell lysates.  Dilutions for western blotting represent a starting point dilution and further optimization may be required.  The antibody detects a band of approximately 96.0 kDa (predicted molecular weight: 72.2 kDa). Specific band detection by western blot is blocked by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane.  Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:10,000 - 1:40,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur WRNIP1

Antigène: WRNIP1 (Werner Helicase Interacting Protein 1 (WRNIP1))

Autre désignation: WRNIP1

Sujet: Background: Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging.  Werner helicase interacting protein 1 (WHIP) interacts with the N-terminal portion of Werner protein, which contains an exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene product may influence the aging process.  A second isoform exists (WHIP2).

ID gène: 56897, 55661735

UniProt: Q96S55