CD28 anticorps (Biotin)

N° du produit ABIN135534

Cet anticorps anti-CD28 est un anticorps Souris Monoclonal détectant CD28 dans FACS. Adapté pour Poulet.

Aperçu rapide pour CD28 anticorps (Biotin) (ABIN135534)

Antigène: CD28

Reactivité: Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD28 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Clone: AV7

Détail du produit anti-CD28 anticorps (Biotin)

Specificité: Chicken/Turkey CD28, Mr 40-44 kDa

Attributs du produit: Mouse Anti-Chicken CD28-BIOT

Immunogène: Chicken peripheral blood lymphocytes

Isotype: IgG1

Information d'application

Indications d'application:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Costim - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Commentaires:

In vitro T-cell costimulation

Volume d'échantillon: 1 mL

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C

Détails sur CD28

Antigène: CD28

Autre désignation: CD28

Sujet: Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral + T cells are CD28-negative.1 Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both V1- and V2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response