CD8B anticorps (FITC)

N° du produit ABIN135542

L’anticorps Souris Monoclonal anti-CD8B a été validé pour FACS. Il convient pour détecter CD8B dans des échantillons de Poulet. Il y a 2+ publications disponibles.

Aperçu rapide pour CD8B anticorps (FITC) (ABIN135542)

Antigène: CD8B (CD8b Molecule (CD8B))

Reactivité: Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8B est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Clone: EP42

Détail du produit anti-CD8B anticorps (FITC)

Specificité: Chicken CD8β, Mr 34 kDa

Attributs du produit: Mouse Anti-Chicken CD8b-FITC

Immunogène: Chicken splenocytes

Isotype: IgG2a

Information d'application

Indications d'application:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Volume d'échantillon: 1 mL

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C

Références pour anticorps anti-CD8B (FITC) (ABIN135542)

Lacharme-Lora, Chaloner, Gilroy, Humphrey, Gibbs, Jopson, Wright, Reid, Ketley, Humphrey, Williams, Rushton, Wigley: "B lymphocytes play a limited role in clearance of Campylobacter jejuni from the chicken intestinal tract." dans: Scientific reports, Vol. 7, pp. 45090, (2018) (PubMed).

Dalgaard, Norup, Pedersen, Handberg, Juorgensen, Juul-Madsen: "Flow cytometric assessment of chicken T cell-mediated immune responses after Newcastle disease virus vaccination and challenge." dans: Vaccine, (2010) (PubMed).

Détails sur CD8B

Antigène: CD8B (CD8b Molecule (CD8B))

Autre désignation: CD8b

Sujet: In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two chains, and (ii) a heterodimer of an chain and a chain.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8- and CD8-chains, a relatively large proportion of the CD8+ TCR- cells in the spleens of embryos and young chicks express only the -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- CD8 cells in the chicken that is not found in rodents.3 Chicken CD8 is expressed on approximately 80 % of thymocytes, 45 % of blood mononuclear cells and 50 % of spleen cells, but <1 % of cells in the bursa and bone marrow.

Pathways: TCR Signaling