SF3B1 anticorps (AA 98-198)

N° du produit ABIN1449241

Détail du produit anti-SF3B1 anticorps

Antigène: SF3B1 (Splicing Factor 3b, Subunit 1, 155kDa (SF3B1))

Épitope: AA 98-198

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SF3B1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificité: This antibody reacts with Sap155.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse

Purification: Protein A agarose

Immunogène: Recombinant GST-Sap155 (98 a.a.- 198 a.a.)

Clone: 16

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2. Contains no preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Upon receipt, store (in aliqouts) at -20 °C.

Références pour anticorps anti-SF3B1 (ABIN1449241)

Salas-Armenteros, Barroso, Rondón, Pérez, Andújar, Luna, Aguilera: "Depletion of the MFAP1/SPP381 Splicing Factor Causes R-Loop-Independent Genome Instability." dans: Cell reports, Vol. 28, Issue 6, pp. 1551-1563.e7, (2020) (PubMed).

Détails sur SF3B1

Antigène: SF3B1 (Splicing Factor 3b, Subunit 1, 155kDa (SF3B1))

Autre désignation: SF3B1 / SAP155 (SF3B1 Produits)

Synonymes: anticorps Cus1, anticorps SAP145, anticorps SF3B145, anticorps SF3b1, anticorps SF3b150, anticorps Hsh155, anticorps MDS, anticorps PRP10, anticorps PRPF10, anticorps SAP155, anticorps SF3b155, anticorps 155kDa, anticorps 2810001M05Rik, anticorps AA409119, anticorps Prp10, anticorps TA-8, anticorps Targ4, anticorps hsh155, anticorps prp10, anticorps prpf10, anticorps sap155, anticorps SF3B1, anticorps Sap155, anticorps wu:fb99f09, anticorps splicing factor 3b subunit 2, anticorps splicing factor 3b subunit 1, anticorps splicing factor 3b, subunit 1, anticorps splicing factor 3b subunit 1 S homeolog, anticorps SF3B2, anticorps SF3B1, anticorps Sf3b1, anticorps sf3b1.S, anticorps sf3b1

Sujet: SF3 is a U2 snRNP-associated protein complex essential for spliceosome assembly and splicing catalysis of the majo r spliceosome. SF3 contains the Spliceosome-Associated Proteins, Sap 49, 130, 145, and 155. Sap155/Sf3b1 is an essential subunit of the U2 snRNP for mRNA splicing and has also been identified in the minor (U12-dependent) sp liceosome. Sap155 interacts with the mammalian PcG (Polycomb group) proteins, Mel18 and Ring1B by the yeast two hybrid system. Sap155 contains numerous Cdk consensus phosphorylation sites in its N terminus and is phosphorylated prior to catal ytic step II of the splicing pathway. Sap155 serves as a substrate for cyclin E-cdk2 in vitro , suggesting that pre-mRNA splicing may be linked to the cell cycle machiner y in mammalian cells.Synonyms: Pre-mRNA-splicing factor SF3b 155 kDa subunit, SAP 155, SAP-155, SF3b155, Spliceosome-associated protein 155, Splicing factor 3B subunit 1

Poids moléculaire: 155 kDa

ID gène: 23451

NCBI Accession: NP_036565

UniProt: O75533

Pathways: Chromatin Binding