RAD9A anticorps (AA 257-306)

N° du produit ABIN1450554

Cet anticorps anti-RAD9A Polyclonal Lapin (ABIN1450554) détecte spécifiquement RAD9A dans ELISA, IHC et IF. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour RAD9A anticorps (AA 257-306) (ABIN1450554)

Antigène: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD9A est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-RAD9A anticorps

Épitope: AA 257-306

Fonction: Rad9 Antibody

Specificité: Rad9 Polyclonal Antibody detects endogenous levels of Rad9 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pureté: > 95 %

Immunogène: The antiserum was produced against synthesized peptide derived from human RAD9. AA range:257-306

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C/1 year

Date de péremption: 12 months

Détails sur RAD9A

Antigène: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Autre désignation: RAD9

Sujet:

Synonyms: RAD9A, Cell cycle checkpoint control protein RAD9A, hRAD9, DNA repair exonuclease rad9 homolog A

Background: catalytic activity:Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.,function:Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds, endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths, and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.,PTM:Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.,similarity:Belongs to the rad9 family.,subunit:Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B, HUS1, RAD1, ABL1, RPA1, ATAD5 and RPA2.,

Gene Name: RAD9A

Protein Name: Cell cycle checkpoint control protein RAD9A

Poids moléculaire: 42 kDa

ID gène: 5883

OMIM: 603761

UniProt: Q96C41

Pathways: Positive Regulation of Response to DNA Damage Stimulus