FEN1 anticorps (AA 86-135)

N° du produit ABIN1533720

Cet anticorps Lapin Polyclonal détecte spécifiquement FEN1 dans WB, IHC, IF et ELISA. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour FEN1 anticorps (AA 86-135) (ABIN1533720)

Antigène: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FEN1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Détail du produit anti-FEN1 anticorps

Épitope: AA 86-135

Fonction: FEN-1 Antibody

Specificité: FEN-1 Polyclonal Antibody detects endogenous levels of FEN-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pureté: > 95 %

Immunogène: The antiserum was produced against synthesized peptide derived from human FEN1. AA range:86-135

Isotype: IgG

Information d'application

Indications d'application: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C/1 year

Date de péremption: 12 months

Détails sur FEN1

Antigène: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Autre désignation: FEN1

Sujet:

Synonyms: FEN1; RAD2; Flap endonuclease 1; FEN-1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1; MF1; hFEN-1

Background: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage byThe protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions

Gene Name: FEN1

Protein Name: Flap endonuclease 1

Poids moléculaire: 42 kDa

ID gène: 2237

OMIM: 600393

UniProt: P39748

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA