ATP6V1B2 anticorps

N° du produit ABIN1573989

Détail du produit anti-ATP6V1B2 anticorps

Antigène: ATP6V1B2 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet, Drosophila melanogaster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATP6V1B2 est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody reacts with human Anti-V-ATPase subunit B. Sequence homology predicts that it will also react with mouse, rat, chicken, bovine, and fruit fly V-ATPase subunit B 2.

Réactivité croisée (Details): Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody reacts with human Anti-V-ATPase subunit B. Sequence homology predicts that it will also react with mouse, rat, chicken, bovine, and fruit fly V-ATPase subunit B 2.

Purification: Immunoaffinity chromatography

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.03-0.125 µg/mL Western blot: 1.0 µg/mL Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS, pH 7.4, containing 30 % glycerol and 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable for 2-3 weeks if stored at 2-8 °C. For long term storage, aliquot and store at -28 °C or below.

Références pour anticorps anti-ATP6V1B2 (ABIN1573989)

Ahmad, Khundmiri, Pribble, Merchant, Ameen, Klein, Levi, Lederer: "Role of vacuolar ATPase in the trafficking of renal type IIa sodium-phosphate cotransporter." dans: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 27, Issue 6, pp. 703-14, (2011) (PubMed).

Détails sur ATP6V1B2

Antigène: ATP6V1B2 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2))

Autre désignation: V-ATPase Subunit B 2 (ATP6V1B2 Produits)

Synonymes: anticorps Atp6b1b2, anticorps Atp6b2, anticorps Vatb, anticorps vha55, anticorps VATB, anticorps ATP6B1B2, anticorps ATP6B2, anticorps HO57, anticorps VPP3, anticorps Vma2, anticorps atp6v1bb, anticorps fj51e01, anticorps vatB2, anticorps wu:fj51e01, anticorps zgc:109771, anticorps AI194269, anticorps AI790362, anticorps R74844, anticorps ATPase H+ transporting V1 subunit B2, anticorps ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 S homeolog, anticorps ATPase, H+ transporting, lysosomal, V1 subunit B2, anticorps ATPase, H+ transporting, lysosomal V1 subunit B2, anticorps Atp6v1b2, anticorps atp6v1b2.S, anticorps ATP6V1B2, anticorps atp6v1b2

Sujet: The V-ATPase subunit B 2 is related to a gene that encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody is developed in rabbit using a synthetic peptide conjugated to KLH. It is purified from rabbit antiserum by immunoaffinity chromatography and supplied as 40 ug aliquot at concentration of 0.5 mg/ml.

Pathways: Transition Metal Ion Homeostasis, Proton Transport