Histone 2b (HIST1H2BL) (C-Term) anticorps

N° du produit ABIN1574024

Détail du produit

Antigène: Histone 2b (HIST1H2BL)

Épitope: C-Term

Reactivité: Humain, Drosophila melanogaster, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB)

Specificité: Rabbit Anti-Histone 2B Polyclonal Antibody detects endogenous levels of human histone 2B protein. Sequence homology predicts that it will also react with Histone 2B protein within mouse and fruit fly proteins.

Réactivité croisée (Details): Rabbit Anti-Histone 2B Polyclonal Antibody detects endogenous levels of human histone 2B protein. Sequence homology predicts that it will also react with Histone 2B protein within mouse and fruit fly proteins.

Purification: Immunoaffinity chromatography

Immunogène: Synthetic peptide (KLH-coupled) derived from the carboxyl terminal of human histone 2B .

Isotype: IgG

Information d'application

Indications d'application: The investigator must determine the working concentrations for each specific application. The appropriate concentration may depend on such factors as secondary antibody affinity, antigen concentration, detection method, temperature, and length of incubation. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-0 °C. For long term storage, aliquot and store at -20 °C or below.

Détail du antigène

Antigène: Histone 2b (HIST1H2BL)

Autre désignation: Histone 2B (HIST1H2BL Produits)

Synonymes: anticorps H2b, anticorps H2B/c, anticorps H2BFC, anticorps dJ97D16.4, anticorps H2b-291a, anticorps histone 2B, anticorps histone cluster 1, H2bl, anticorps histone cluster 1 H2B family member l, anticorps histone H2B type 1, anticorps PVX_090935, anticorps HTB2101, anticorps Hist1h2bl, anticorps HIST1H2BL, anticorps LOC100295221

Sujet: Histone 2B is the core component of nucleosome. It is involved in nucleosome wrapping and the compaction of DNA into chromatin, limiting DNA accessibility. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex system of nucleosome remodelling and post-translational modification of histones, also called histone code.Rabbit Anti-Histone 2B Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide corresponding to carboxyl terminal residues of human histone 2B protein (Swiss Prot: Q99879).