Fibrillarin anticorps (AA 50-100)

N° du produit ABIN1574130

Détail du produit anti-Fibrillarin anticorps

Antigène: Fibrillarin (FBL)

Épitope: AA 50-100

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibrillarin est non-conjugé

Application: Western Blotting (WB)

Specificité: Rabbit Anti-Fibrillarin Polyclonal Antibody detects endogenous levels of human Fibrillarin.Positive Control: Hela and HEK293.

Purification: Immunoaffinity chromatography

Immunogène: KLH-coupled synthetic peptide within AA 50-100 of human Fibrillarin protein .

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Références pour anticorps anti-Fibrillarin (ABIN1574130)

Berkovits, Wolgemuth: "The first bromodomain of the testis-specific double bromodomain protein Brdt is required for chromocenter organization that is modulated by genetic background." dans: Developmental biology, Vol. 360, Issue 2, pp. 358-68, (2011) (PubMed).

Détails sur Fibrillarin

Antigène: Fibrillarin (FBL)

Autre désignation: Fibrillarin (FBL Produits)

Synonymes: anticorps FIB, anticorps FLRN, anticorps RNU3IP1, anticorps wu:fb37g12, anticorps zgc:56145, anticorps zgc:77130, anticorps FBL, anticorps AL022665, anticorps CG9888, anticorps Dmel\\CG9888, anticorps Fibri, anticorps GCR-6, anticorps GCR6, anticorps Pen59C5, anticorps fib, anticorps pen59C5, anticorps MGC76139, anticorps fbl, anticorps fibrillarin, anticorps Fibrillarin, anticorps putative fibrillarin, anticorps fibrillarin S homeolog, anticorps FBL, anticorps fbl, anticorps Fbl, anticorps Fib, anticorps fib1, anticorps LMJF_19_0100, anticorps fbl.S

Sujet: Fibrillarin is an excellent marker for the nucleolus. As a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle, it is thought to participate in the first step in processing preribosomal RNA. Fibrillarin protein contains an amino-terminal domain that is rich in glycine and arginine residues (termed the GAR domain), a central RNA-binding domain comprising an RNP-2 like consensus sequence, and a C-terminal alpha-helical domain. Fibrillarin binds to U3, U8, and U13 small nuclear RNAs. It associates with several other structural proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. This complex catalyzes site-specific 2-O-ribose methylation of targeted nucleotides within the rRNA sequence. Rabbit Anti-Fibrillarin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 50-100 of human Fibrillarin protein (Swiss Prot: P22087).

Pathways: Ribonucleoside Biosynthetic Process