MAPKAP Kinase 2 anticorps (Internal Region)

N° du produit ABIN1590006

Cet anticorps anti-MAPKAP Kinase 2 est un anticorps Chèvre Polyclonal détectant MAPKAP Kinase 2 dans WB, IHC, ELISA et IP. Adapté pour Humain et Souris.

Aperçu rapide pour MAPKAP Kinase 2 anticorps (Internal Region) (ABIN1590006)

Antigène: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp MAPKAP Kinase 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Classe de qualité: Verified

Détail du produit anti-MAPKAP Kinase 2 anticorps

Épitope: Internal Region

Fonction: MK2 / MAPKAPK2

Séquence: SRVLKEDKER WED

Specificité: This antibody is expected to recognize both reported isoforms (NP_004750.1, NP_116584.2).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60 kDa band observed in Mouse Embryonic Fibroblasts (MEF) lysates, not present in the knockout MEFs and reappearing as a 45 kDa band upon reintroduction of MK2 gene expression through viral transduction (calculated MW of 44.0 kDa accord

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunoprecipitation: MK2/MK3 double knockout MEFs retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were labelled with rabbit anti-MK2

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur MAPKAP Kinase 2

Antigène: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Autre désignation: MAPKAPK2

Sujet: MAPKAPK2, mitogen-activated protein kinase-activated protein kinase 2, MAPKAP-K2, MK-2, MK2, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2

ID gène: 9261, 17164, 289014

NCBI Accession: NP_004750, NP_116584

Pathways: Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades