MAPKAP Kinase 5 anticorps (Internal Region)

N° du produit ABIN1590011

L’anticorps Chèvre Polyclonal anti-MAPKAP Kinase 5 a été validé pour WB, ELISA, IHC et IP. Il convient pour détecter MAPKAP Kinase 5 dans des échantillons de Humain et Souris.

Aperçu rapide pour MAPKAP Kinase 5 anticorps (Internal Region) (ABIN1590011)

Antigène: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp MAPKAP Kinase 5 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Classe de qualité: Verified

Détail du produit anti-MAPKAP Kinase 5 anticorps

Épitope: Internal Region

Fonction: MK5 / MAPKAPK5

Séquence: STEALDNVLP SAQ

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Kidney shows brushborder staining in the PCT. Recommended concentration, 4-8 μg/mL.

Western Blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP, only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemi

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur MAPKAP Kinase 5

Antigène: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Autre désignation: Mapkapk5

Sujet: MAPKAPK5, mitogen-activated protein kinase-activated protein kinase 5, MAPKAP-K5, MK-5, MK5, PRAK, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, p38-regulated/activated protein kinase

ID gène: 8550, 17165, 498183

NCBI Accession: NP_003659, NP_620777

Pathways: Signalisation MAPK