GLB1 anticorps (Tag) (Biotin)

N° du produit ABIN1607765

Détail du produit anti-GLB1 anticorps (Biotin)

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Épitope: Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLB1 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity against β-Galactosidase from other sources may occur but have not been specifically determined.

Pureté: Anti-BETA GALACTOSIDASE Antibody, Biotin Conjugated, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified beta-Galactosidase [E.coli].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Beta Galactosidase (E.coli)
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.  The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:5.000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus.  A 1:1.500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ.  In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 µm) to produce free-floating sections.  A 1:5.000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy.  A 1:5.000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos.  Although a wide range of conditions was reported to be effective, a 1:10.000 dilution was noted to show no background and to be suitable for double labeling experiments.  Optimal titers for other applications should be determined by the researcher.
ELISA Dilution: 1:10.000 - 1:50.000
Western Blot Dilution: 1:2.000 - 1:10.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur GLB1

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Autre désignation: Beta Galactosidase (GLB1 Produits)

Synonymes: anticorps EBP, anticorps ELNR1, anticorps MPS4B, anticorps DER, anticorps XR, anticorps 0610038K04Rik, anticorps 1810027P18Rik, anticorps glb, anticorps CG9092, anticorps Dmel\\CG9092, anticorps beta-GAL, anticorps beta-Gal-1, anticorps beta-gal, anticorps gal, anticorps lacZ-1, anticorps AW125515, anticorps Bge, anticorps Bgl, anticorps Bgl-e, anticorps Bgl-s, anticorps Bgl-t, anticorps Bgs, anticorps Bgt, anticorps C130097A14Rik, anticorps BGAL, anticorps zgc:110823, anticorps ECK0341, anticorps JW0335, anticorps GLB, anticorps galactosidase beta 1, anticorps dicarbonyl and L-xylulose reductase, anticorps dicarbonyl L-xylulose reductase, anticorps beta-D-galactosidase, anticorps beta-galactosidase, anticorps beta galactosidase, anticorps galactosidase, beta 1, anticorps beta-galactosidase-like, anticorps GLB1, anticorps DCXR, anticorps Dcxr, anticorps lacZ, anticorps SSP_RS00525, anticorps lacH, anticorps ECL_03691, anticorps Gal, anticorps Glb1, anticorps glb1, anticorps VV2_1327, anticorps LOC100056371

Sujet: Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations.

Pathways: Glycosaminoglycan Metabolic Process