Hexokinase anticorps (Biotin)

N° du produit ABIN1607791

Détail du produit anti-Hexokinase anticorps (Biotin)

Antigène: Hexokinase

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Hexokinase est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity against Hexokinase from other sources may occur but have not been specifically determined.

Pureté: Anti-Hexokinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Hexokinase [Yeast]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Hexokinase has been assayed against 1.0 µg of Hexokinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:4.000 - 1:20.000
Western Blot Dilution: 1:500 - 1:2.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur Hexokinase

Antigène: Hexokinase

Abstract: Hexokinase Produits

Synonymes: anticorps CG32849, anticorps CG5443, anticorps Dmel\\CG32849, anticorps HK, anticorps Hex, anticorps anon-WO0140519.203, anticorps CG8094, anticorps CT22069, anticorps DM2, anticorps Dmel\\CG8094, anticorps Fk, anticorps HEX, anticorps HEX-3, anticorps HEX-C, anticorps Hex-3, anticorps Hex-c, anticorps HexC, anticorps Hk, anticorps CG3001, anticorps DM1, anticorps Dmel\\CG3001, anticorps HEX-A, anticorps Hex-A:B, anticorps HexA, anticorps CG33102, anticorps CG54430, anticorps DHK-453, anticorps Dmel\\CG33102, anticorps HEX-t1, anticorps Dyak\\GE17799, anticorps GE17799, anticorps Hex-A, anticorps dyak_GLEANR_19098, anticorps Dyak\\GE12255, anticorps GE12255, anticorps Hex-C, anticorps dyak_GLEANR_12524, anticorps dsim_GLEANR_11078, anticorps DsimGD11104, anticorps GD11104, anticorps CG32849 gene product from transcript CG32849-RA, anticorps Hexokinase C, anticorps Hexokinase A, anticorps CG33102 gene product from transcript CG33102-RA, anticorps Phosphotransferase, anticorps GE17799 gene product from transcript GE17799-RA, anticorps GE12255 gene product from transcript GE12255-RA, anticorps GD11104 gene product from transcript GD11104-RA, anticorps hexokinase, anticorps Hex-t2, anticorps Hex-C, anticorps Hex-A, anticorps Hex-t1, anticorps hxk-1, anticorps Dyak\Hex-A, anticorps Dyak\Hex-C, anticorps Dsim\Hex-C, anticorps Smp_043030

Sujet: Anti-Hexokinase antibody recognizes hexokinase which phosphorylates hexoses. Hexokinase uses one ATP molecule when phosphorylating six-carbon sugars. In mammals there are four isozymes of hexokinase, the most notable of which is hexokinase IV, also known as glucokinase which is highly specific for glucose. Phosporylation of hexoses commit them to certain metabolic pathways. For example, phosphorylation of glucose to glucose-6-phosphate limits it to glycolysis or glycogen synthesis. Anti-Hexokinase antibody is ideal for investigators interested in Metabolism, Cardiology, or Enzymology. Synonyms: DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody

ID gène: 852639

UniProt: P04807