Aspartate Transaminase anticorps (Biotin)

N° du produit ABIN1607837

Détail du produit anti-Aspartate Transaminase anticorps (Biotin)

Antigène: Aspartate Transaminase (Ast)

Reactivité: Porc

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp Aspartate Transaminase est conjugé à/à la Biotin

Application: ELISA, Western Blotting (WB)

Réactivité croisée (Details): Cross reactivity against Aspartate Aminotransferase (AST) from other sources may occur but has not been specifically determined.

Pureté: Anti-Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Aspartate Aminotransferase (AST) [Pig Heart]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Aspartate Transaminase has been assayed against 1.0 µg of Aspartate Aminotransferase (AST in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Aspartate Aminotransferase (AST in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur Aspartate Transaminase

Antigène: Aspartate Transaminase (Ast)

Autre désignation: Aspartate Transaminase (Ast Produits)

Synonymes: anticorps Afu6g02490, anticorps Afu2g09650, anticorps aspartate transaminase, anticorps Aspartate transaminase, anticorps Aspartate transaminase, putative, anticorps aspB, anticorps CNM00360, anticorps AFUA_6G02490, anticorps AFUA_2G09650, anticorps AAT_1, anticorps Dd586_1627, anticorps aspB3, anticorps Fbal_1917, anticorps Emin_0749, anticorps CGB_M0340W, anticorps Sgly_0435, anticorps Weevi_1216, anticorps Selsp_1595, anticorps Trebr_2143

Sujet: Anti-Aspartate Transaminase antibody is present in the cytoplasm of cells, belonging to the class-I pyridoxal-phosphate-dependent aminotransferase family. Playing an active role in amino acid metabolism, Anti-Aspartate Transaminase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction research. Synonyms: Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody

ID gène: 396967

UniProt: P00503