CD11b anticorps (APC)

N° du produit ABIN1690733

Détail du produit anti-CD11b anticorps (APC)

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivité: Humain, Souris, Lapin, Non-Human Primate

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11b est conjugé à/à la APC

Application: Flow Cytometry (FACS)

Specificité: The rat monoclonal antibody M1/70 detects an extracellular epitope of CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.

Réactivité croisée (Details): Human, Non-Human Primates, Mouse, Rabbit

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: B10 mouse spleen cells enriched for T cells

Clone: M1-70

Isotype: IgG2b

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1 μg/mL.

Commentaires:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD11b (APC) (ABIN1690733)

Takagi, Numazaki, Kajiwara, Abe, Ishii, Kato, Kojima: "Cooperation of specific ICAM-3 grabbing nonintegrin-related 1 (SIGNR1) and complement receptor type 3 (CR3) in the uptake of oligomannose-coated liposomes by macrophages." dans: Glycobiology, Vol. 19, Issue 3, pp. 258-66, (2009) (PubMed).

Leon, Contractor, Fuss, Marth, Lahey, Iwaki, la Sala, Hoffmann, Strober, Kelsall: "Antibodies to complement receptor 3 treat established inflammation in murine models of colitis and a novel model of psoriasiform dermatitis." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 10, pp. 6974-82, (2006) (PubMed).

Brickson, Ji, Schell, Olabisi, St Pierre Schneider, Best: "M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury." dans: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 95, Issue 3, pp. 969-76, (2003) (PubMed).

Zhang, McCormick, Desai, Wu, Gilliam: "Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 6, pp. 3088-98, (2002) (PubMed).

Welt, Edelman, Simon, Rogers: "Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries." dans: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 12, pp. 2553-8, (2000) (PubMed).

Dembic, Schenck, Bogen: "Dendritic cells purified from myeloma are primed with tumor-specific antigen (idiotype) and activate CD4+ T cells." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 6, pp. 2697-702, (2000) (PubMed).

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, (1995) (PubMed).

Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." dans: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, (1983) (PubMed).

Ault, Springer: "Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 359-64, (1981) (PubMed).

Springer, Galfrè, Secher, Milstein: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." dans: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, (1978) (PubMed).

Détails sur CD11b

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Autre désignation: CD11b (ITGAM Produits)

Synonymes: anticorps CD11b/CD18, anticorps CR3, anticorps CR3A, anticorps Cd11b, anticorps F730045J24Rik, anticorps Ly-40, anticorps MAC1, anticorps Mac-1, anticorps Mac-1a, anticorps CD11B, anticorps MAC-1, anticorps MAC1A, anticorps MO1A, anticorps SLEB6, anticorps C3bi, anticorps integrin subunit alpha M, anticorps integrin alpha M, anticorps integrin, alpha M (complement component 3 receptor 3 subunit), anticorps integrin alpha-M, anticorps ITGAM, anticorps Itgam, anticorps LOC100351865

Sujet: Integrin subunit alpha M,CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.,Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A

ID gène: 16409

UniProt: G5E8F1

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation