ATF2 anticorps (pThr51, pThr53)

N° du produit ABIN1713945

Cet anticorps anti-ATF2 est un anticorps Lapin Polyclonal détectant ATF2 dans WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) et ICC. Adapté pour Humain.

Aperçu rapide pour ATF2 anticorps (pThr51, pThr53) (ABIN1713945)

Antigène: ATF2 (Activating Transcription Factor 2 (ATF2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATF2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Détail du produit anti-ATF2 anticorps

Épitope: pThr51, pThr53

Réactivité croisée: Humain

Homologie: Mouse,Rat

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from mouse ATF2 around the phosphorylation site of Thr51 + Thr53

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur ATF2

Antigène: ATF2 (Activating Transcription Factor 2 (ATF2))

Autre désignation: ATF2 +

Sujet:

Synonyms: ATF2 phospho T51 + T53, p-ATF2 phospho T51 + T53, ATF2 phospho Thr51 + Thr53, p-ATF2 phospho Thr51 + Thr53, CREB 2, HB 16,Activating Transcription Factor 2, ATF 2, Atf-2, ATF2 protein, cAMP Response Element Binding Protein 2, cAMP response element binding protein CRE BP1, cAMP-dependent transcription factor ATF-2, cAMP-responsive element-binding protein 2, CRE BP1, CRE-BP, CREB 2, CREB2, CREBP1, Cyclic AMP dependent transcription factor ATF 2, Cyclic AMP-responsive, ATF2_HUMAN.

Background: ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

ID gène: 11909

Pathways: Signalisation MAPK, Signalisation RTK, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades