Interleukin 8 anticorps (IL8)

Details for Product anti-IL8 Antibody No. ABIN1724413, Fournisseur: Connectez-vous pour afficher
Antigène
  • CXCL8
  • GCP-1
  • GCP1
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
  • MONAP
  • NAF
  • NAP-1
  • NAP1
  • IL-8
  • AMCF-I
  • il8
  • IL8
  • si:dkey-151b16.2
  • cxcli2
  • cxcl8
  • gcp-1
  • il-8
  • lynap
  • mdncf
  • monap
  • nap-1
  • C-X-C motif chemokine ligand 8
  • interleukin 8
  • chemokine (C-X-C motif) ligand 8a
  • chemokine (C-X-C motif) ligand 1-like
  • chemokine (C-X-C motif) ligand 8 L homeolog
  • CXCL8
  • il8
  • cxcl8a
  • IL8
  • Cxcl8
  • LOC422654
  • cxcl8.L
Reactivité
Humain
405
32
30
26
20
19
17
16
15
11
10
7
6
5
4
2
1
1
1
1
Hôte
Souris
304
248
11
6
6
6
2
Clonalité
Monoclonal
Conjugué
Cet anticorp Interleukin 8 est non-conjugé
73
56
16
14
10
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
393
297
180
104
50
47
44
34
33
26
23
21
15
12
9
6
4
3
2
2
2
2
2
2
2
1
1
1
1
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
'Independent Validation' signe
  • Testé avec succès (Immunohistochemistry)
  • by  HIK Hannover
  • No. #100789
  • Date 31.05.2017
  •  
Antigène IL8
Application validée Immunohistochemistry
Contrôle positif Formalin fixed and plastic embedded human liver, testicle, and tonsil tissue sections
Contrôle négative No primary antibody
Conclusion Passed. The IL8 antibody ABIN1724413 specifically labels the targeted antigen in human liver, testicle, and tonsil sections in IHC. No signal was detected in tonsil negative control tissue sections and the no primary antibody negative controls.
Anticorps primaire ABIN1724413
Anticorps secondaire Histofine Simple Stain MAX PO (M) Anti-Mouse (Medac Diagnostika, 414132F, lot no H1608)
Protocole
  • Disect tissue and fix in 4% buffered formalin for >24h at RT.
  • Plastification in Technovit 9100 plastic embedding system (Heraeus Kulzer) according to manufactuer’s recommencations.
  • Sectioning into 5µm sections on a rotary microtome (Reichert Jung, 1140/Autocut).
  • Deplastify and rehydrate tissue through graded xylene and graded alcohol series:
    • xylene for 20min.
    • MEA (2-Methoxyethyl)acetat zur Synthese (Merck, 8.06061.1000) 2x 20min.
    • 100% ethanol for 1min.
    • 95% ethanol for 1min.
    • 70% ethanol for 1min.
    • H2O for 2min.
    • distilled H2O 2min.
  • Heat induced epitope retrieval in high pH Trilogy buffer (Medac Diagnostika, 920P-07, lot 1622912A) ON at 60°C in an oven (Memmert, UNB 200).
  • Quench sections in in ice cold distilled H2O 2min.
  • Adjust sections with wash buffer (Medac Diagnostika, B1-30A, lot no 161102001) 5min at RT.
  • Incubate sections with primary Interleukin 8 antibody (antibodies-online, ABIN1724413) diluted 1:400 in dilution buffer (Medac Diagnostika, B1-31C, lot no 161205001) for 30min at RT. Includeno primaryantibody negative controls.
  • Rinse sections 3x 5min with wash buffer. Keep negative controls in a separate container.
  • Block endogenous peroxidase with Peroxide Block RTU (Medac Diagnostika, 925B-09, lot no 161103001) for 10min at RT.
  • Rinse sections 3x 5min with wash buffer.
  • Incubate sections with Histofine Simple Stain MAX PO (M) Anti-Mouse (Medac Diagnostika, 414132F, lot no H1608) for 30min at RT.
  • Rinse sections 3x 5min with wash buffer.
  • Incubate sections with Bright DAB (Medac Diagnostika, BS04-110, lot no 160523) for 10min at RT.
  • Rinse sections in distilled H2O.
  • Counterstain with Mayers Haematoxylin (Leica, 3801582E, lot no3801582E) 5min at RT.
  • Dehydrate in fresh ethanol:
    • 95% ethanol twice for 2min.
    • 99.5% ethanol twice for 2min.
    • Xylene twice for 2min.
  • Mount sections with Celltexx mounting medium (Engelbrecht, 19360, lot no 1716047).
  • Dry sections in a ventilated place.
  • Image acquisition on a Leica Microsystems DM 1000 light microscope with a DFC 320 digital camera. Magnification tonsil sections 40x, liver sections 10x and 20x, testicle sections 10x.
Notes Immunostaining pattern of human liver, testicle, and tonsil with ABIN1724413 is consistent with published data.
Validation Images
Immunohistochemistry validation image for anti-Interleukin 8 (IL8) antibody (ABIN1724413) Immunostaining of IL8 in human liver liver (A), testicle (C), and tonsil sections (D)...
Immunogène Recombinant protein corresponding to full length IL8
Isotype IgG1
Specificité Reacts with human IL-8
Purification Purified
Plasmids, Primers & others Plasmids, Primers & others Interleukin 8 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation IL-8 (IL8 Antibody Extrait)
Sujet IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
ID gène 3576
Pathways Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy
Indications d'application

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
IHC-P (5 μg/mL) WB (1:1000)

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Citrate-Tris-HCl buffer, pH 7.0, 0.02 % Proclin 300
Agent conservateur ProClin
Précaution d'utilisation This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C/-20 °C
Stockage commentaire Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Images (Fournisseur)
 image for anti-Interleukin 8 (IL8) antibody (ABIN1724413) anti-Interleukin 8 (IL8) antibody
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