APAF1 anticorps (C-Term)

N° du produit ABIN1731833

L’anticorps anti-APAF1 Polyclonal Lapin est utilisé pour la détection de APAF1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC, ELISA, ICC et IF.

Aperçu rapide pour APAF1 anticorps (C-Term) (ABIN1731833)

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp APAF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Détail du produit anti-APAF1 anticorps

Épitope: AA 1158-1177, C-Term

Specificité: Reacts with human, mouse, and rat Apaf1 (130 kDa).

Purification: Purified

Immunogène: Synthetic peptide, corresponding to amino acids 1158-1177 (C-terminus) of Human Apaf 1. The sequence of the immunogenic peptide differs from that of murine Apaf1 byone amino acid

Isotype: IgG

Information d'application

Indications d'application: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
IHC(10 - 20 μg/mL) WB(1 - 10 μg/mL) ELISA(1:100 - 1:2000)

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS, pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute in PBS or medium that is identical to that used in the assay system.

Stock: -20 °C

Stockage commentaire: Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.

Date de péremption: 12 months

Détails sur APAF1

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Autre désignation: Apaf1

Sujet: The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis proteaseactivating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.

Apoptotic protease-activating factor 1, APAF-1 Gene name: APAF1, KIAA0413, CED4

ID gène: 317

UniProt: O14727

Pathways: Signalisation p53, Apoptose, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity