Synthetic peptide, corresponding to amino acids 12-28 of Human Apaf 1. The sequence of the immunogenic peptide differs from that of murine Apaf1 by one amino acid.
APAF1
Reactivité: Humain
WB, IF
Hôte: Souris
Monoclonal
24-Apaf
unconjugated
Indications d'application
Working dilution: Optimal dilution should be determined by the end user. The following are guidelines only: IHC(1 - 2 μg/mL) IF (10 μg/mL) WB(1 - 2 μg/mL)
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS, pH 7.4
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Dilute in PBS or medium that is identical to that used in the assay system.
Stock
-20 °C
Stockage commentaire
Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.
The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis proteaseactivating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.