UNC13A/Munc13-1 anticorps (AA 3-317)

N° du produit ABIN1742277

Détail du produit anti-UNC13A/Munc13-1 anticorps

Antigène: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Épitope: AA 3-317

Reactivité: Humain, Souris, Rat, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UNC13A/Munc13-1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Specificité: Specific for munc 13-1.

Purification: antiserum

Immunogène: Recombinant rat munc 13-1 (aa 3-317).

Information d'application

Indications d'application: WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC: 1 : 200
ELISA: not tested yet

Commentaires:

IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: This antibody gives much better results in ICC than the monoclonal antibody. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742276 as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.

Buffer: PBS

Conseil sur la manipulation: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Stock: 4 °C/-20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Références pour anticorps anti-UNC13A/Munc13-1 (ABIN1742277)

Fernandes, Riordan, Nomura, Remmers, Kraniotis, Marshall, Kukreja, Vassar, Contractor: "Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 16, pp. 6544-53, (2015) (PubMed).

Juranek, Mukherjee, Siddiqui, Kaplan, Li, Ahnert-Hilger, Jahn, Calka: "Active zone protein expression changes at the key stages of cerebellar cortex neurogenesis in the rat." dans: Acta histochemica, Vol. 115, Issue 6, pp. 616-25, (2013) (PubMed).

Rinetti, Schweizer: "Ubiquitination acutely regulates presynaptic neurotransmitter release in mammalian neurons." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 9, pp. 3157-66, (2010) (PubMed).

Détails sur UNC13A/Munc13-1

Antigène: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Autre désignation: Munc 13-1 (UNC13A Produits)

Synonymes: anticorps Munc13-1, anticorps Unc13h1, anticorps 2410078G03Rik, anticorps unc-13 homolog A, anticorps unc-13 homolog A (C. elegans), anticorps Unc13a, anticorps UNC13A

Pathways: Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis